BMRB Entry 50221

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50221

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Title:
Staphylococcus aureus EF-GC3 methyl side chain chemical shifts
Deposition date:
2020-04-01
Original release date:
2020-10-07
Authors:
Tomlinson, Jennifer; Kalverda, Arnout
Citation:

Citation: Tomlinson, Jennifer; Kalverda, Arnout; Calabrese, Antonio. "Fusidic acid resistance through changes in the dynamics of the drug target"  Proc. Natl. Acad. Sci. U. S. A. 117, 25523-25531 (2020).
PubMed: 32999060

Assembly members:

Assembly members:
entity_1, polymer, 301 residues, 33424.6284 Da.

Natural source:

Natural source:   Common Name: Staphylococcus aureus   Taxonomy ID: 1280   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Staphylococcus aureus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-29b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MEFPEPVIHLSVEPKSKADQ DKMTQALVKLQEEDPTFHAH TDEETGQVIIGGMGELHLDI LVDRMKKEFNVECNVGAPMV SYRETFKSSAQVQGKFSRQS GGRGQYGDVHIEFTPNETGA GFEFENAIVGGVVPREYIPS VEAGLKDAMENGVLAGYPLI DVKAKLYDGSYHDVDSSEMA FKIAASLALKEAAKKCDPVI LEPMMKVTIEMPEEYMGDIM GDVTSRRGRVDGMEPRGNAQ VVNAYVPLSEMFGYATSLRS NTQGRGTYTMYFDHYAEVPK SIAEDIIKKNKGELEHHHHH H

Data sets:
Data typeCount
13C chemical shifts265
1H chemical shifts361

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1EF-GC31

Entities:

Entity 1, EF-GC3 301 residues - 33424.6284 Da.

This truncated protein begins at residue 401 of the full length EF-G protein and comprises domains III-V of EF-G. Residues 694-701 represent a non-native affinity tag

1   METGLUPHEPROGLUPROVALILEHISLEU
2   SERVALGLUPROLYSSERLYSALAASPGLN
3   ASPLYSMETTHRGLNALALEUVALLYSLEU
4   GLNGLUGLUASPPROTHRPHEHISALAHIS
5   THRASPGLUGLUTHRGLYGLNVALILEILE
6   GLYGLYMETGLYGLULEUHISLEUASPILE
7   LEUVALASPARGMETLYSLYSGLUPHEASN
8   VALGLUCYSASNVALGLYALAPROMETVAL
9   SERTYRARGGLUTHRPHELYSSERSERALA
10   GLNVALGLNGLYLYSPHESERARGGLNSER
11   GLYGLYARGGLYGLNTYRGLYASPVALHIS
12   ILEGLUPHETHRPROASNGLUTHRGLYALA
13   GLYPHEGLUPHEGLUASNALAILEVALGLY
14   GLYVALVALPROARGGLUTYRILEPROSER
15   VALGLUALAGLYLEULYSASPALAMETGLU
16   ASNGLYVALLEUALAGLYTYRPROLEUILE
17   ASPVALLYSALALYSLEUTYRASPGLYSER
18   TYRHISASPVALASPSERSERGLUMETALA
19   PHELYSILEALAALASERLEUALALEULYS
20   GLUALAALALYSLYSCYSASPPROVALILE
21   LEUGLUPROMETMETLYSVALTHRILEGLU
22   METPROGLUGLUTYRMETGLYASPILEMET
23   GLYASPVALTHRSERARGARGGLYARGVAL
24   ASPGLYMETGLUPROARGGLYASNALAGLN
25   VALVALASNALATYRVALPROLEUSERGLU
26   METPHEGLYTYRALATHRSERLEUARGSER
27   ASNTHRGLNGLYARGGLYTHRTYRTHRMET
28   TYRPHEASPHISTYRALAGLUVALPROLYS
29   SERILEALAGLUASPILEILELYSLYSASN
30   LYSGLYGLULEUGLUHISHISHISHISHIS
31   HIS

Samples:

sample_1: EF-GC3, [U-100% 13C; U-100% 15N; U-80% 2H], 0.25 mM; TrisHCl 20 mM; NaCl 300 mM; DTT 1 mM

sample_conditions_1: ionic strength: 0.310 M; pH: 8.000; pressure: 1.000 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CCH-TOCSYsample_1isotropicsample_conditions_1

Software:

CcpNmr_Analysis v2.1 - assignment

NMRPipe - processing

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Bruker Avance 750 MHz

Related Database Links:

NCBI P68790