BMRB Entry 50216

Name: HR1c domain from PRK1
Published: 2020-06-09

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50216

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

HR1c domain from PRK1

Deposition date:

2020-03-25

Original release date:

2020-06-09

Authors:

Sophocleous, Georgios; Mott, Helen

Citation:

Citation: Sophocleous, Georgios; Wood, George; Owen, Darerca; Mott, Helen. "1 H, 15 N and 13 C Resonance Assignments of the HR1c Domain of PRK1, a Protein Kinase C-related Kinase" Biomol. NMR Assignments 14, 245-250 (2020).
PubMed: 32500230

Assembly members:

Assembly members:
entity_1, polymer, 104 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGex6P

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GPLGSHMDTQGSPDLGAVEL RIEELRHHFRVEHAVAEGAK NVLRLLSAAKAPDRKAVSEA QEKLTESNQKLGLLREALER RLGELPADHPKGRLLREELA AASS

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	346
15N chemical shifts	102
1H chemical shifts	730

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PRK1 HR1c	1

Entities:

Entity 1, PRK1 HR1c 104 residues - Formula weight is not available

1

GLY

PRO

LEU

GLY

SER

HIS

MET

ASP

THR

GLN

2

GLY

SER

PRO

ASP

LEU

GLY

ALA

VAL

GLU

LEU

3

ARG

ILE

GLU

LEU

ARG

HIS

PHE

ARG

4

VAL

GLU

HIS

ALA

VAL

ALA

GLU

GLY

ALA

LYS

5

ASN

VAL

LEU

ARG

LEU

SER

ALA

LYS

6

ALA

PRO

ASP

ARG

LYS

ALA

VAL

SER

GLU

ALA

7

GLN

GLU

LYS

LEU

THR

GLU

SER

ASN

GLN

LYS

8

LEU

GLY

LEU

ARG

GLU

ALA

LEU

GLU

ARG

9

ARG

LEU

GLY

GLU

LEU

PRO

ALA

ASP

HIS

PRO

10

LYS

GLY

ARG

LEU

ARG

GLU

LEU

ALA

11

ALA

SER

Samples:

sample_1: PRK1 HR1c domain, [U-100% 15N], 1.6 mM; sodium phosphate 20 mM; sodium chloride 150 mM

sample_2: PRK1 HR1c domain, [U-100% 13C; U-100% 15N], 1.2 mM; sodium phosphate 20 mM; sodium chloride 150 mM

sample_conditions_1: ionic strength: 0.17 M; pH: 7.3; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_2	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_2	isotropic	sample_conditions_1
3D HNCA	sample_2	isotropic	sample_conditions_1
3D HNCACB	sample_2	isotropic	sample_conditions_1
3D HN(CO)CA	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_2	isotropic	sample_conditions_1

Software:

CcpNmr Analysis v2.4 - chemical shift assignment

NMR spectrometers:

Bruker DRX 500 MHz
Bruker Avance 800 MHz

UNP	Q16512
AlphaFold	Q9UD44