BMRB Entry 50215
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50215
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Citation: Ludzia, Patryk; Akiyoshi, Bungo; Redfield, Christina. "1 H, 13 C and 15 N resonance assignments for the microtubule-binding domain of the kinetoplastid kinetochore protein KKT4 from Trypanosoma brucei" Biomol. NMR Assignments 14, 309-315 (2020).
PubMed: 32696260
Assembly members:
entity_1, polymer, 62 residues, 7152.14 Da.
Natural source: Common Name: Trypanosoma brucei Taxonomy ID: 5691 Superkingdom: Eukaryota Kingdom: not available Genus/species: Trypanosoma brucei
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pRSF_Duet-1
Entity Sequences (FASTA):
entity_1: SMKYGVVSVERYERLMARYK
ELEKQSHRRQGKRSEPVVDT
QRVLDLEEEVARLKRTIGHL
QG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 218 |
| 15N chemical shifts | 63 |
| 1H chemical shifts | 325 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | KKT4_115_174, monomer 1 | 1 |
| 2 | KKT4_115_174, monomer 2 | 1 |
Entities:
Entity 1, KKT4_115_174, monomer 1 62 residues - 7152.14 Da.
Residues 1-2 (113S, 114M) represent part of a remained linker after TEV protease cleavage
| 1 | SER | MET | LYS | TYR | GLY | VAL | VAL | SER | VAL | GLU | ||||
| 2 | ARG | TYR | GLU | ARG | LEU | MET | ALA | ARG | TYR | LYS | ||||
| 3 | GLU | LEU | GLU | LYS | GLN | SER | HIS | ARG | ARG | GLN | ||||
| 4 | GLY | LYS | ARG | SER | GLU | PRO | VAL | VAL | ASP | THR | ||||
| 5 | GLN | ARG | VAL | LEU | ASP | LEU | GLU | GLU | GLU | VAL | ||||
| 6 | ALA | ARG | LEU | LYS | ARG | THR | ILE | GLY | HIS | LEU | ||||
| 7 | GLN | GLY |
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