BMRB Entry 50214

Title:
1HN and 15N assignment of PhoQ PD at pH 6.5
Deposition date:
2020-03-18
Original release date:
2020-04-21
Authors:
Hicks, Kevin; Scott, Delbecq; Enea, Sancho-Vaello; Marie-Pierre, Blanc; Katja, Dove; Lynne, Prost; Margaret, Daley; Kornelius, Zeth; Rachel, Klevit; Samuel, Miller
Citation:

Citation: Hicks, Kevin; Delbecq, Scott; Sancho-Vaello, Enea; Blanc, Marie-Pierre; Dove, Katja; Prost, Lynne; Daley, Margaret; Zeth, Kornelius; Klevit, Rachel; Miller, Samuel. "Acidic pH and divalent cation sensing by PhoQ are dispensable for systemic salmonellae virulence."  Elife 4, e06792-e06792 (2015).
PubMed: 26002083

Assembly members:

Assembly members:
entity_1, polymer, 153 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Salmonella typhimurium   Taxonomy ID: 28901   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Salmonella enterica

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11a-phoQ 45-190-(His)6

Data sets:
Data typeCount
15N chemical shifts125
1H chemical shifts125

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PhoQ_PD1

Entities:

Entity 1, PhoQ_PD 153 residues - Formula weight is not available

1   METASPLYSTHRTHRPHEARGLEULEUARG
2   GLYGLUSERASNLEUPHETYRTHRLEUALA
3   LYSTRPGLUASNASNLYSILESERVALGLU
4   LEUPROGLUASNLEUASPMETGLNSERPRO
5   THRMETTHRLEUILETYRASPGLUTHRGLY
6   LYSLEULEUTRPTHRGLNARGASNILEPRO
7   TRPLEUILELYSSERILEGLNPROGLUTRP
8   LEULYSTHRASNGLYPHEHISGLUILEGLU
9   THRASNVALASPALATHRSERTHRLEULEU
10   SERGLUASPHISSERALAGLNGLULYSLEU
11   LYSGLUVALARGGLUASPASPASPASPALA
12   GLUMETTHRHISSERVALALAVALASNILE
13   TYRPROALATHRALAARGMETPROGLNLEU
14   THRILEVALVALVALASPTHRILEPROILE
15   GLULEULYSARGSERTYRMETHISHISHIS
16   HISHISHIS

Samples:

sample_1: PhoQ_PD, [U-100% 15N], 400 uM; sodium phosphate 20 mM; sodium chloride 150 mM; magnesium chloride 20 mM; EDTA 20 mM

sample_conditions_1: ionic strength: 0.2 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

CcpNmr Analysis - data analysis

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

UNP D0ZV89.1
AlphaFold D0ZV89

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks