BMRB Entry 50213

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments of the yeast TIM8/13 complex
Published: 2020-04-02

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50213

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments of the yeast TIM8/13 complex

Deposition date:

2020-03-18

Original release date:

2020-04-02

Authors:

Sucec, Iva; Weinhaupl, Katharina; Hessel, Audrey; Bersch, Beate; Schanda, Paul

Citation:

Citation: Sucec, Iva; Wang, Yong; Dakhlaoui, Ons; Weinhaupl, Katharina; Jores, Tobias; Costa, Doriane; Hessel, Audrey; Brennich, Martha; Rapaport, Doron; Lindorff-Larsen, Kresten; Bersch, Beate; Schanda, Paul. "Structural basis of client specificity in mitochondrial membrane-protein chaperones" Sci. Adv. 6, eabd0263-eabd0263 (2020).
PubMed: 33355130

Assembly members:

Assembly members:
entity_1, polymer, 87 residues, Formula weight is not available
entity_2, polymer, 105 residues, Formula weight is not available

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET_Duet1_Tim8_Tim13

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MSSLSTSDLASLDDTSKKEI ATFLEGENSKQKVQMSIHQF TNICFKKCVESVNDSNLSSQ EEQCLSNCVNRFLDTNIRIV NGLQNTR
entity_2: GGLSSIFGGGAPSQQKEAAT TAKTTPNPIAKELKNQIAQE LAVANATELVNKISENCFEK CLTSPYATRNDACIDQCLAK YMRSWNVISKAYISRIQNAS ASGEI

Data sets:

assigned_chemical_shifts

Data type	Count
13C chemical shifts	685
15N chemical shifts	293
1H chemical shifts	293

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Tim8	1
2	Tim13	2

Entities:

Entity 1, Tim8 87 residues - Formula weight is not available

1

MET

SER

LEU

SER

THR

SER

ASP

LEU

ALA

2

SER

LEU

ASP

THR

SER

LYS

GLU

ILE

3

ALA

THR

PHE

LEU

GLU

GLY

GLU

ASN

SER

LYS

4

GLN

LYS

VAL

GLN

MET

SER

ILE

HIS

GLN

PHE

5

THR

ASN

ILE

CYS

PHE

LYS

CYS

VAL

GLU

6

SER

VAL

ASN

ASP

SER

ASN

LEU

SER

GLN

7

GLU

GLN

CYS

LEU

SER

ASN

CYS

VAL

ASN

8

ARG

PHE

LEU

ASP

THR

ASN

ILE

ARG

ILE

VAL

9

ASN

GLY

LEU

GLN

ASN

THR

ARG

Entity 2, Tim13 105 residues - Formula weight is not available

1

GLY

LEU

SER

ILE

PHE

GLY

2

ALA

PRO

SER

GLN

LYS

GLU

ALA

THR

3

THR

ALA

LYS

THR

PRO

ASN

PRO

ILE

ALA

4

LYS

GLU

LEU

LYS

ASN

GLN

ILE

ALA

GLN

GLU

5

LEU

ALA

VAL

ALA

ASN

ALA

THR

GLU

LEU

VAL

6

ASN

LYS

ILE

SER

GLU

ASN

CYS

PHE

GLU

LYS

7

CYS

LEU

THR

SER

PRO

TYR

ALA

THR

ARG

ASN

8

ASP

ALA

CYS

ILE

ASP

GLN

CYS

LEU

ALA

LYS

9

TYR

MET

ARG

SER

TRP

ASN

VAL

ILE

SER

LYS

10

ALA

TYR

ILE

SER

ARG

ILE

GLN

ASN

ALA

SER

11

ALA

SER

GLY

GLU

ILE

Samples:

sample_1: Tim8, [U-13C; U-15N; U-2H], 0.15 mM; Tim13, [U-13C; U-15N; U-2H], 0.15 mM

sample_2: Tim8, [U-13C; U-15N; U-2H], 0.1 mM; Tim13 0.1 mM

sample_conditions_1: pH: 7.4; temperature: 308 K

sample_conditions_2: pH: 7.4; temperature: 333 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N BEST-TROSY HSQC	sample_1	isotropic	sample_conditions_1
3D BEST-TROSY HNCO	sample_1	isotropic	sample_conditions_1
3D BEST-TROSY HNCA	sample_1	isotropic	sample_conditions_1
3D BEST-TROSY HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D BEST-TROSY HN(COCA)CB	sample_1	isotropic	sample_conditions_1
3D BEST-TROSY HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D BEST-TROSY HN(CA)CB	sample_1	isotropic	sample_conditions_1
2D 1H-15N BEST-TROSY HSQC	sample_1	isotropic	sample_conditions_2
3D BEST-TROSY HNCO	sample_1	isotropic	sample_conditions_2
3D BEST-TROSY HNCA	sample_1	isotropic	sample_conditions_2
3D BEST-TROSY HN(CO)CA	sample_1	isotropic	sample_conditions_2
3D BEST-TROSY HN(COCA)CB	sample_1	isotropic	sample_conditions_2
3D BEST-TROSY HN(CA)CO	sample_1	isotropic	sample_conditions_2
3D BEST-TROSY HN(CA)CB	sample_1	isotropic	sample_conditions_2
2D 1H-15N BEST-TROSY HSQC	sample_2	isotropic	sample_conditions_1

Software:

CcpNmr Analysis - chemical shift assignment

TOPSPIN - collection

NMR spectrometers:

Bruker Avance 700 MHz
Bruker Avance 950 MHz