BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 50213

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments of the yeast TIM8/13 complex

Deposition date: 2020-03-18 Original release date: 2020-04-02

Authors: Sucec, Iva; Weinhaupl, Katharina; Hessel, Audrey; Bersch, Beate; Schanda, Paul

Citation: Sucec, Iva; Weinhaupl, Katharina; Hessel, Audrey; Bersch, Beate; Schanda, Paul. "Backbone 1H, 13C, and 15N Chemical Shift Assignments of the yeast TIM8/13 complex"  .

Assembly members:
entity_1, polymer, 87 residues, Formula weight is not available
entity_2, polymer, 105 residues, Formula weight is not available

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET_Duet1_Tim8_Tim13

Entity Sequences (FASTA):
entity_1: MSSLSTSDLASLDDTSKKEI ATFLEGENSKQKVQMSIHQF TNICFKKCVESVNDSNLSSQ EEQCLSNCVNRFLDTNIRIV NGLQNTR
entity_2: GGLSSIFGGGAPSQQKEAAT TAKTTPNPIAKELKNQIAQE LAVANATELVNKISENCFEK CLTSPYATRNDACIDQCLAK YMRSWNVISKAYISRIQNAS ASGEI

Data typeCount
13C chemical shifts685
15N chemical shifts293
1H chemical shifts293

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Tim81
2Tim132

Entities:

Entity 1, Tim8 87 residues - Formula weight is not available

1   METSERSERLEUSERTHRSERASPLEUALA
2   SERLEUASPASPTHRSERLYSLYSGLUILE
3   ALATHRPHELEUGLUGLYGLUASNSERLYS
4   GLNLYSVALGLNMETSERILEHISGLNPHE
5   THRASNILECYSPHELYSLYSCYSVALGLU
6   SERVALASNASPSERASNLEUSERSERGLN
7   GLUGLUGLNCYSLEUSERASNCYSVALASN
8   ARGPHELEUASPTHRASNILEARGILEVAL
9   ASNGLYLEUGLNASNTHRARG

Entity 2, Tim13 105 residues - Formula weight is not available

1   GLYGLYLEUSERSERILEPHEGLYGLYGLY
2   ALAPROSERGLNGLNLYSGLUALAALATHR
3   THRALALYSTHRTHRPROASNPROILEALA
4   LYSGLULEULYSASNGLNILEALAGLNGLU
5   LEUALAVALALAASNALATHRGLULEUVAL
6   ASNLYSILESERGLUASNCYSPHEGLULYS
7   CYSLEUTHRSERPROTYRALATHRARGASN
8   ASPALACYSILEASPGLNCYSLEUALALYS
9   TYRMETARGSERTRPASNVALILESERLYS
10   ALATYRILESERARGILEGLNASNALASER
11   ALASERGLYGLUILE

Samples:

sample_1: Tim8, [U-13C; U-15N; U-2H], 0.15 mM; Tim13, [U-13C; U-15N; U-2H], 0.15 mM

sample_2: Tim8, [U-13C; U-15N; U-2H], 0.1 mM; Tim13 0.1 mM

sample_conditions_1: pH: 7.4; temperature: 308 K

sample_conditions_2: pH: 7.4; temperature: 333 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N BEST-TROSY HSQCsample_1isotropicsample_conditions_1
3D BEST-TROSY HNCOsample_1isotropicsample_conditions_1
3D BEST-TROSY HNCAsample_1isotropicsample_conditions_1
3D BEST-TROSY HN(CO)CAsample_1isotropicsample_conditions_1
3D BEST-TROSY HN(COCA)CBsample_1isotropicsample_conditions_1
3D BEST-TROSY HN(CA)COsample_1isotropicsample_conditions_1
3D BEST-TROSY HN(CA)CBsample_1isotropicsample_conditions_1
2D 1H-15N BEST-TROSY HSQCsample_1isotropicsample_conditions_2
3D BEST-TROSY HNCOsample_1isotropicsample_conditions_2
3D BEST-TROSY HNCAsample_1isotropicsample_conditions_2
3D BEST-TROSY HN(CO)CAsample_1isotropicsample_conditions_2
3D BEST-TROSY HN(COCA)CBsample_1isotropicsample_conditions_2
3D BEST-TROSY HN(CA)COsample_1isotropicsample_conditions_2
3D BEST-TROSY HN(CA)CBsample_1isotropicsample_conditions_2
2D 1H-15N BEST-TROSY HSQCsample_2isotropicsample_conditions_1

Software:

CcpNmr Analysis - chemical shift assignment

TOPSPIN - collection

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker Avance 950 MHz

Related Database Links:

PDB

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts