BMRB Entry 50210

Title:
EAP34
Deposition date:
2020-03-12
Original release date:
2021-07-27
Authors:
Pal, Indrani; Prakash, Om
Citation:

Citation: Pal, Indrani; Mishra, Nitin; Herrera, Alvaro; Dubey, Abhinav; Arthanari, Haribabu; Geisbrecht, Brian; Prakash, Om. "1H, 15N, and 13C backbone resonance assignments of the C4b-binding region from the S. aureus extracellular adherence protein"  Biomol. NMR Assign. 15, 183-186 (2021).
PubMed: 33423171

Assembly members:

Assembly members:
entity_1, polymer, 208 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Staphylococcus aureus   Taxonomy ID: 1280   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Staphylococcus aureus

Experimental source:

Experimental source:   Production method: obtained from a collaborator   Host organism: Escherichia coli

Data sets:
Data typeCount
13C chemical shifts581
15N chemical shifts199
1H chemical shifts199

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1EAP341

Entities:

Entity 1, EAP34 208 residues - Formula weight is not available

1   VALPROTYRSERILEASNLEUASNGLYTHR
2   SERTHRASNILELEUSERASNLEUSERPHE
3   SERASNLYSPROTRPTHRASNTYRLYSASN
4   LEUTHRSERGLNILELYSSERVALLEULYS
5   HISASPARGGLYILESERGLUGLNASPLEU
6   LYSTYRALALYSLYSALATYRTYRTHRVAL
7   TYRPHELYSASNGLYGLYLYSARGILELEU
8   GLNLEUASNSERLYSASNTYRTHRALAASN
9   LEUVALHISALALYSASPVALLYSARGILE
10   GLUILETHRVALLYSTHRGLYTHRLYSALA
11   LYSALAASPARGTYRVALPROTYRTHRILE
12   ALAVALASNGLYTHRSERTHRPROILELEU
13   SERLYSLEULYSILESERASNLYSGLNLEU
14   ILESERTYRLYSTYRLEUASNASPLYSVAL
15   LYSSERVALLEULYSSERGLUARGGLYILE
16   SERASPLEUASPLEULYSPHEALALYSGLN
17   ALALYSTYRTHRVALTYRPHELYSASNGLY
18   LYSLYSGLNVALVALASNLEULYSSERASP
19   ILEPHETHRPROASNLEUPHESERALALYS
20   ASPILELYSLYSILEASPILEASPVALLYS
21   GLNTYRTHRLYSSERLYSLYSLYS

Samples:

sample_1: Extracellular Adherence Protein, [U-100% 13C; U-100% 15N], 800 uM

sample_conditions_1: pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

CARA - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks