BMRB Entry 50207

Name: Purification of native CCL7 and its functional interaction with selected chemokine receptors
Published: 2020-04-06

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50207

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Purification of native CCL7 and its functional interaction with selected chemokine receptors

Deposition date:

2020-03-07

Original release date:

2020-04-06

Authors:

Goncharuk, Marina; Roy, Debarati; Dubinnyi, Maxim; Nadezhdin, Kirill; Srivastava, Ashish; Baidya, Mithu; Dwivedi-Agnihotri, Hemlata; Arseniev, Alexander; Shukla, Arun

Citation:

Citation: Goncharuk, Marina; Roy, Debarati; Dubinnyi, Maxim; Nadezhdin, Kirill; Srivastava, Ashish; Baidya, Mithu; Dwivedi-Agnihotri, Hemlata; Arseniev, Alexander; Shukla, Arun. "Purification of native CCL7 and its functional interaction with selected chemokine receptors" Protein Expr. Purif. 171, 105617-105617 (2020).
PubMed: 32145391

Assembly members:

Assembly members:
entity_1, polymer, 76 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEMEX-1(his6)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: QPVGINTSTTCCYRFINKKI PKQRLESYRRTTSSHCPREA VIFKTKLDKEICADPTQKWV QDFMKHLDKKTQTPKL

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
15N chemical shifts	80
1H chemical shifts	513

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	natCCL7	1

Entities:

Entity 1, natCCL7 76 residues - Formula weight is not available

1

GLN

PRO

VAL

GLY

ILE

ASN

THR

SER

THR

2

CYS

TYR

ARG

PHE

ILE

ASN

LYS

ILE

3

PRO

LYS

GLN

ARG

LEU

GLU

SER

TYR

ARG

4

THR

SER

HIS

CYS

PRO

ARG

GLU

ALA

5

VAL

ILE

PHE

LYS

THR

LYS

LEU

ASP

LYS

GLU

6

ILE

CYS

ALA

ASP

PRO

THR

GLN

LYS

TRP

VAL

7

GLN

ASP

PHE

MET

LYS

HIS

LEU

ASP

LYS

8

THR

GLN

THR

PRO

LYS

LEU

Samples:

sample_1: Native human chemokine CCL7, [U-100% 15N], 0.45 mM; KPi 20 mM; MES 0.4 mM; NaCl 7 mM

sample_conditions_1: ionic strength: 0.007 M; pH: 5.1; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
1D 1H	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v3.0 - collection

CARA v1.9.1.7 - chemical shift assignment

NMR spectrometers:

Bruker Avance 700 MHz
Bruker Avance III 800 MHz

SP	P80098
AlphaFold	Q569J6