BMRB Entry 50204

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50204

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Title:
Musashi-1 C-terminal domain, residues 194-362
Deposition date:
2020-02-24
Original release date:
2020-06-09
Authors:
Chen, Tsai-chen; Huang, Jie-rong
Citation:

Citation: Chen, Tsai-chen; Huang, Jie-rong. "Musashi-1: An Example of How Polyalanine Tracts Contribute to Self-Association in the Intrinsically Disordered Regions of RNA-Binding Proteins"  Int. J. Mol. Sci. 21, 2289-2289 (2020).
PubMed: 32225071

Assembly members:

Assembly members:
Musashi-1, polymer, 169 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Musashi-1: GSARGRSRVMPYGMDAFMLG IGMLGYPGFQATTYASRSYT GLAPGYTYQFPEFRVERSPL PSAPVLPELTAIPLTAYGPM AAAAAAAAVVRGTGSHPWTM APPPGSTPSRTGGFLGTTSP GPMAELYGAANQDSGVSSYI SAASPAPSTGFGHSLGGPLI ATAFTNGYH

Data sets:
Data typeCount
13C chemical shifts462
15N chemical shifts145
1H chemical shifts145

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Musashi-11

Entities:

Entity 1, Musashi-1 169 residues - Formula weight is not available

1   GLYSERALAARGGLYARGSERARGVALMET
2   PROTYRGLYMETASPALAPHEMETLEUGLY
3   ILEGLYMETLEUGLYTYRPROGLYPHEGLN
4   ALATHRTHRTYRALASERARGSERTYRTHR
5   GLYLEUALAPROGLYTYRTHRTYRGLNPHE
6   PROGLUPHEARGVALGLUARGTHRPROLEU
7   PROSERALAPROVALLEUPROGLULEUTHR
8   ALAILEPROLEUTHRALATYRGLYPROMET
9   ALAALAALAALAALAALAALAALAVALVAL
10   ARGGLYTHRGLYSERHISPROTRPTHRMET
11   ALAPROPROPROGLYSERTHRPROSERARG
12   THRGLYGLYPHELEUGLYTHRTHRSERPRO
13   GLYPROMETALAGLULEUTYRGLYALAALA
14   ASNGLNASPSERGLYVALSERSERTYRILE
15   SERALAALASERPROALAPROSERTHRGLY
16   PHEGLYHISSERLEUGLYGLYPROLEUILE
17   ALATHRALAPHETHRASNGLYTYRHIS

Samples:

sample_1: Musashi-1, [U-99% 15N], 20-100 uM

sample_conditions_1: pH: 5.5; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D (H)N(COCO)NHsample_1isotropicsample_conditions_1
3D (HN)CO(CO)NHsample_1isotropicsample_conditions_1

Software:

SPARKY - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks