BMRB Entry 50196

Name: Backbone chemical shifts of E2A residues 1-100
Published: 2020-02-24

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50196

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone chemical shifts of E2A residues 1-100

Deposition date:

2020-02-13

Original release date:

2020-02-24

Authors:

Langelaan, David

Citation:

Citation: Lochhead, Marina; Brown, Alexandra; Kirlin, Alyssa; Chitayat, Seth; Munro, Kim; Findlay, Jane; Baillie, George; LeBrun, David; Langelaan, David; Smith, Steven. "Structural insights into TAZ2 domain-mediated CBP/p300 recruitment by transactivation domain 1 of the lymphopoietic transcription factor E2A" J. Biol. Chem. 295, 4303-4315 (2020).
PubMed: 32098872

Assembly members:

Assembly members:
entity_1, polymer, 102 residues, 10486.3478 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET 21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSMNQPQRMAPVGTDKELSD LLDFSMMFPLPVTNGKGRPA SLAGAQFGGSGLEDRPSSGS WGSGDQSSSSFDPSRTFSEG THFTESHSSLSSSTFLGPGL GG

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1
spectral_peak_list
- spectral_peak_list_1

Data type	Count
13C chemical shifts	295
15N chemical shifts	93
1H chemical shifts	140

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	E2A:1-100	1

Entities:

Entity 1, E2A:1-100 102 residues - 10486.3478 Da.

1

GLY

SER

MET

ASN

GLN

PRO

GLN

ARG

MET

ALA

2

PRO

VAL

GLY

THR

ASP

LYS

GLU

LEU

SER

ASP

3

LEU

ASP

PHE

SER

MET

PHE

PRO

LEU

4

PRO

VAL

THR

ASN

GLY

LYS

GLY

ARG

PRO

ALA

5

SER

LEU

ALA

GLY

ALA

GLN

PHE

GLY

SER

6

GLY

LEU

GLU

ASP

ARG

PRO

SER

GLY

SER

7

TRP

GLY

SER

GLY

ASP

GLN

SER

8

PHE

ASP

PRO

SER

ARG

THR

PHE

SER

GLU

GLY

9

THR

HIS

PHE

THR

GLU

SER

HIS

SER

LEU

10

SER

THR

PHE

LEU

GLY

PRO

GLY

LEU

11

GLY

Samples:

sample_1: entity_1, [U-13C; U-15N], 1 mM; D2O, [U-100% 2H], 5 % v/v; sodium chloride 50 mM; MES 20 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC/HMQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

CcpNmr Analysis v2.4 - collection, data analysis, processing

NMR spectrometers:

Varian INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks