BMRB Entry 50193

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50193

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Structures of the free and bound forms of the intrinsically disordered plant viral genome-linked protein
Deposition date:
2020-02-07
Original release date:
2021-08-12
Authors:
Dixit, Karuna; Karanth, Megha; Nair, Smita; Kumari, Khushboo; Chakraborti, Kalyan; Savitri, H.; Sarma, Siddhartha
Citation:

Citation: Dixit, Karuna; Karanth, Megha; Nair, Smita; Kumari, Khushboo; Chakraborti, Kalyan; Savitri, H.; Sarma, Siddhartha. "Aromatic Interactions Drive the Coupled Folding and Binding of the Intrinsically Disordered Sesbania mosaic Virus VPg Protein"  Biochemistry 59, 4663-4680 (2020).
PubMed: 33269926

Assembly members:

Assembly members:
entity_1, polymer, 296 residues, Formula weight is not available
entity_2, polymer, 57 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pRSET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MRGSHHHHHHGMASSLKLNV GDASNESAVLGSFYSPVKAG DEPASLVAIKSGPTTIGFGC RTKIDGEDCLLTAHHVWCNS MRPTGLAKAGKQVSVEDWEI SMSSSDKMLDFAIVRVPTHV WSKLGVKSTPLVCPSSKDVI TCYGGSSSDCLMSGVGSSST SEFTWKLTHTCPTAAGWAGT PLYSSRGVVGMHVGFEEIGK LNRGVNMFYVANYLLRSNET LPPELSIIEIPFDDVETRSY EFIEVEIKGRGKAKLGKREF AWIPESGKYWADEDEDELPP PPKLVGGKLVWENAQE
entity_2: GSMTLPPELSIIEIPFDDVE TRSYEFIEVEIKGRGKAKLG KREFAWIPESGKYWADE

Data sets:
Data typeCount
13C chemical shifts152
15N chemical shifts49
1H chemical shifts85

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ProVPg1
2VPg2

Entities:

Entity 1, ProVPg 296 residues - Formula weight is not available

Karuna Dixit, N. Megha Karanth, Smita Nair, Khushboo Kumari, Kalyan S. Chakraborti, H.S. Savithri, and Siddhartha P Sarma.

1   METARGGLYSERHISHISHISHISHISHIS
2   GLYMETALASERSERLEULYSLEUASNVAL
3   GLYASPALASERASNGLUSERALAVALLEU
4   GLYSERPHETYRSERPROVALLYSALAGLY
5   ASPGLUPROALASERLEUVALALAILELYS
6   SERGLYPROTHRTHRILEGLYPHEGLYCYS
7   ARGTHRLYSILEASPGLYGLUASPCYSLEU
8   LEUTHRALAHISHISVALTRPCYSASNSER
9   METARGPROTHRGLYLEUALALYSALAGLY
10   LYSGLNVALSERVALGLUASPTRPGLUILE
11   SERMETSERSERSERASPLYSMETLEUASP
12   PHEALAILEVALARGVALPROTHRHISVAL
13   TRPSERLYSLEUGLYVALLYSSERTHRPRO
14   LEUVALCYSPROSERSERLYSASPVALILE
15   THRCYSTYRGLYGLYSERSERSERASPCYS
16   LEUMETSERGLYVALGLYSERSERSERTHR
17   SERGLUPHETHRTRPLYSLEUTHRHISTHR
18   CYSPROTHRALAALAGLYTRPALAGLYTHR
19   PROLEUTYRSERSERARGGLYVALVALGLY
20   METHISVALGLYPHEGLUGLUILEGLYLYS
21   LEUASNARGGLYVALASNMETPHETYRVAL
22   ALAASNTYRLEULEUARGSERASNGLUTHR
23   LEUPROPROGLULEUSERILEILEGLUILE
24   PROPHEASPASPVALGLUTHRARGSERTYR
25   GLUPHEILEGLUVALGLUILELYSGLYARG
26   GLYLYSALALYSLEUGLYLYSARGGLUPHE
27   ALATRPILEPROGLUSERGLYLYSTYRTRP
28   ALAASPGLUASPGLUASPGLULEUPROPRO
29   PROPROLYSLEUVALGLYGLYLYSLEUVAL
30   TRPGLUASNALAGLNGLU

Entity 2, VPg 57 residues - Formula weight is not available

Karuna Dixit, N. Megha Karanth, Smita Nair, Khushboo Kumari, Kalyan S. Chakraborti, H.S. Savithri, and Siddhartha P Sarma.

1   GLYSERMETTHRLEUPROPROGLULEUSER
2   ILEILEGLUILEPROPHEASPASPVALGLU
3   THRARGSERTYRGLUPHEILEGLUVALGLU
4   ILELYSGLYARGGLYLYSALALYSLEUGLY
5   LYSARGGLUPHEALATRPILEPROGLUSER
6   GLYLYSTYRTRPALAASPGLU

Samples:

sample_1: entity_1, [U-100% 13C; U-100% 15N; U-80% 2H], 0.3 ± 0.02 mM; entity_2, [U-100% 13C; U-100% 15N; U-80% 2H], 0.3 ± 0.01 mM; potassium phosphate buffer 20 mM; NaCl 50 mM; EDTA 1 mM; sodium azide 0.01%

sample_conditions_1: ionic strength: 0.065 M; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

Analysis, CCPN - data analysis

CcpNMR, CCPN - data analysis

NMR spectrometers:

  • Agilent AMX 600 MHz
  • Bruker AMX 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks