BMRB Entry 50187

Name: RBM5 RRM1-Zf1
Published: 2020-06-09

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50187

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

RBM5 RRM1-Zf1

Deposition date:

2020-02-02

Original release date:

2020-06-09

Authors:

Soni, Komal; Sattler, Michael

Citation:

Citation: Soni, Komal; Martinez-Lumbreras, Santiago; Sattler, Michael. "Conformational Dynamics From Ambiguous Zinc Coordination in the RanBP2-Type Zinc Finger of RBM5" J. Mol. Biol. 432, 4127-4138 (2020).
PubMed: 32450081

Assembly members:

Assembly members:
entity_1, polymer, . residues, Formula weight is not available
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MGERESKTIMLRGLPTTITE SDIREMMESFEGPQPADVRL MKRKTGVSRGFAFVEFYHLQ DATSWMEANQKKLVIQGKHI AMHYSNPRPKFEDWLCNKCC LNNFRKRLKCFRCGADKFD

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	321
15N chemical shifts	110
1H chemical shifts	109

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RRM1-Zf1	1
2	Zn	2

Entities:

Entity 1, RRM1-Zf1 - Formula weight is not available

Sequence numbering starts from 92 and residues 92, 93 are cloning artifacts.

1

MET

GLY

GLU

ARG

GLU

SER

LYS

THR

ILE

MET

2

LEU

ARG

GLY

LEU

PRO

THR

ILE

THR

GLU

3

SER

ASP

ILE

ARG

GLU

MET

GLU

SER

PHE

4

GLU

GLY

PRO

GLN

PRO

ALA

ASP

VAL

ARG

LEU

5

MET

LYS

ARG

LYS

THR

GLY

VAL

SER

ARG

GLY

6

PHE

ALA

PHE

VAL

GLU

PHE

TYR

HIS

LEU

GLN

7

ASP

ALA

THR

SER

TRP

MET

GLU

ALA

ASN

GLN

8

LYS

LEU

VAL

ILE

GLN

GLY

LYS

HIS

ILE

9

ALA

MET

HIS

TYR

SER

ASN

PRO

ARG

PRO

LYS

10

PHE

GLU

ASP

TRP

LEU

CYS

ASN

LYS

CYS

11

LEU

ASN

PHE

ARG

LYS

ARG

LEU

LYS

CYS

12

PHE

ARG

CYS

GLY

ALA

ASP

LYS

PHE

ASP

Entity 2, Zn - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: entity_1, [U-13C; U-15], mM; MES 20 mM; sodium chloride 400 mM; DTT 1 mM

sample_conditions_1: ionic strength: 0.4 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

Analysis, CCPN - chemical shift assignment

NMR spectrometers:

Bruker Avance 800 MHz
Bruker Avance 600 MHz