BMRB Entry 50185

Name: AcrIIA5
Published: 2020-06-03

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50185

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

AcrIIA5

Deposition date:

2020-01-29

Original release date:

2020-06-03

Authors:

Suh, Jeong-Yong

Citation:

Citation: An, So Young; Ka, Donghyun; Kim, Iktae; Kim, Eun-Hee; Kim, Nak-Kyoon; Bae, Euiyoung; Suh, Jeong-Yong. "Intrinsic disorder is essential for Cas9 inhibition of anti-CRISPR AcrIIA5" Nucleic Acids Res. 48, 7584-7594 (2020).
PubMed: 32544231

Assembly members:

Assembly members:
Anti-CRISPR_AcrIIA5, polymer, 140 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Streptococcus thermophilus Taxonomy ID: 1308 Superkingdom: Bacteria Kingdom: not available Genus/species: Streptococcus thermophilus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Anti-CRISPR_AcrIIA5: MAYGKSRYNSYRKRSFNRSN KQRREYAQEMDRLEKAFENL DGWYLSSMKDSAYKDFGKYE IRLSNHSADNKYHDLENGRL IVNIKASKLNFVDIIENKLD KIIEKIDKLDLDKYRFINAT NLEHDIKCYYKGFKTKKEVI

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1_chemical_shifts

Data type	Count
13C chemical shifts	533
15N chemical shifts	121
1H chemical shifts	795

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	AcrIIA5	1

Entities:

Entity 1, AcrIIA5 140 residues - Formula weight is not available

1	MET	ALA	TYR	GLY	LYS	SER	ARG	TYR	ASN	SER
2	TYR	ARG	LYS	ARG	SER	PHE	ASN	ARG	SER	ASN
3	LYS	GLN	ARG	ARG	GLU	TYR	ALA	GLN	GLU	MET
4	ASP	ARG	LEU	GLU	LYS	ALA	PHE	GLU	ASN	LEU
5	ASP	GLY	TRP	TYR	LEU	SER	SER	MET	LYS	ASP
6	SER	ALA	TYR	LYS	ASP	PHE	GLY	LYS	TYR	GLU
7	ILE	ARG	LEU	SER	ASN	HIS	SER	ALA	ASP	ASN
8	LYS	TYR	HIS	ASP	LEU	GLU	ASN	GLY	ARG	LEU
9	ILE	VAL	ASN	ILE	LYS	ALA	SER	LYS	LEU	ASN
10	PHE	VAL	ASP	ILE	ILE	GLU	ASN	LYS	LEU	ASP
11	LYS	ILE	ILE	GLU	LYS	ILE	ASP	LYS	LEU	ASP
12	LEU	ASP	LYS	TYR	ARG	PHE	ILE	ASN	ALA	THR
13	ASN	LEU	GLU	HIS	ASP	ILE	LYS	CYS	TYR	TYR
14	LYS	GLY	PHE	LYS	THR	LYS	LYS	GLU	VAL	ILE

Samples:

sample_1_AcrIIA5: entity_1, [U-100% 13C; U-100% 15N], 0.7 mM

sample_conditions_1: ionic strength: 0.5 M; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-13C NOESY	sample_1_AcrIIA5	isotropic	sample_conditions_1

Software:

PIPP, Garrett - chemical shift assignment

NMR spectrometers:

Bruker Avance 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

1	MET	ALA	TYR	GLY	LYS	SER	ARG	TYR	ASN	SER
2	TYR	ARG	LYS	ARG	SER	PHE	ASN	ARG	SER	ASN
3	LYS	GLN	ARG	ARG	GLU	TYR	ALA	GLN	GLU	MET
4	ASP	ARG	LEU	GLU	LYS	ALA	PHE	GLU	ASN	LEU
5	ASP	GLY	TRP	TYR	LEU	SER	SER	MET	LYS	ASP
6	SER	ALA	TYR	LYS	ASP	PHE	GLY	LYS	TYR	GLU
7	ILE	ARG	LEU	SER	ASN	HIS	SER	ALA	ASP	ASN
8	LYS	TYR	HIS	ASP	LEU	GLU	ASN	GLY	ARG	LEU
9	ILE	VAL	ASN	ILE	LYS	ALA	SER	LYS	LEU	ASN
10	PHE	VAL	ASP	ILE	ILE	GLU	ASN	LYS	LEU	ASP
11	LYS	ILE	ILE	GLU	LYS	ILE	ASP	LYS	LEU	ASP
12	LEU	ASP	LYS	TYR	ARG	PHE	ILE	ASN	ALA	THR
13	ASN	LEU	GLU	HIS	ASP	ILE	LYS	CYS	TYR	TYR
14	LYS	GLY	PHE	LYS	THR	LYS	LYS	GLU	VAL	ILE

1	MET	ALA	TYR	GLY	LYS	SER	ARG	TYR	ASN	SER
2	TYR	ARG	LYS	ARG	SER	PHE	ASN	ARG	SER	ASN
3	LYS	GLN	ARG	ARG	GLU	TYR	ALA	GLN	GLU	MET
4	ASP	ARG	LEU	GLU	LYS	ALA	PHE	GLU	ASN	LEU
5	ASP	GLY	TRP	TYR	LEU	SER	SER	MET	LYS	ASP
6	SER	ALA	TYR	LYS	ASP	PHE	GLY	LYS	TYR	GLU
7	ILE	ARG	LEU	SER	ASN	HIS	SER	ALA	ASP	ASN
8	LYS	TYR	HIS	ASP	LEU	GLU	ASN	GLY	ARG	LEU
9	ILE	VAL	ASN	ILE	LYS	ALA	SER	LYS	LEU	ASN
10	PHE	VAL	ASP	ILE	ILE	GLU	ASN	LYS	LEU	ASP
11	LYS	ILE	ILE	GLU	LYS	ILE	ASP	LYS	LEU	ASP
12	LEU	ASP	LYS	TYR	ARG	PHE	ILE	ASN	ALA	THR
13	ASN	LEU	GLU	HIS	ASP	ILE	LYS	CYS	TYR	TYR
14	LYS	GLY	PHE	LYS	THR	LYS	LYS	GLU	VAL	ILE

1	MET	ALA	TYR	GLY	LYS	SER	ARG	TYR	ASN	SER
2	TYR	ARG	LYS	ARG	SER	PHE	ASN	ARG	SER	ASN
3	LYS	GLN	ARG	ARG	GLU	TYR	ALA	GLN	GLU	MET
4	ASP	ARG	LEU	GLU	LYS	ALA	PHE	GLU	ASN	LEU
5	ASP	GLY	TRP	TYR	LEU	SER	SER	MET	LYS	ASP
6	SER	ALA	TYR	LYS	ASP	PHE	GLY	LYS	TYR	GLU
7	ILE	ARG	LEU	SER	ASN	HIS	SER	ALA	ASP	ASN
8	LYS	TYR	HIS	ASP	LEU	GLU	ASN	GLY	ARG	LEU
9	ILE	VAL	ASN	ILE	LYS	ALA	SER	LYS	LEU	ASN
10	PHE	VAL	ASP	ILE	ILE	GLU	ASN	LYS	LEU	ASP
11	LYS	ILE	ILE	GLU	LYS	ILE	ASP	LYS	LEU	ASP
12	LEU	ASP	LYS	TYR	ARG	PHE	ILE	ASN	ALA	THR
13	ASN	LEU	GLU	HIS	ASP	ILE	LYS	CYS	TYR	TYR
14	LYS	GLY	PHE	LYS	THR	LYS	LYS	GLU	VAL	ILE