BMRB Entry 50183

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50183

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Title:
Cdc42 bound to GDP (AMPylation at Y32)
Deposition date:
2020-01-28
Original release date:
2020-05-30
Authors:
Kang, Hyun-Seo; Barthelmes, Katja; Sattler, Michael
Citation:

Citation: Barthelmes, Katja; Ramcke, Evelyn; Kang, Hyun-Seo; Sattler, Michael; Itzen, Aymelt. "Conformational Control of Small GTPases by AMPylation"  Proc. Natl. Acad. Sci. U.S.A. 117, 5772-5781 (2020).
PubMed: 32123090

Assembly members:

Assembly members:
entity_1_Cdc42, polymer, 188 residues, Formula weight is not available
entity_GDP, non-polymer, 443.201 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pOPINM

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1_Cdc42: HMQTIKCVVVGDGAVGKTCL LISYTTNKFPSEYVPTVFDN YAVTVMIGGEPYTLGLFDTA GLEDYDRLRPLSYPQTDVFL VCFSVVSPSSFENVKEKWVP EITHHCPKTPFLLVGTQIDL RDDPSTIEKLAKNKQKPITP ETAEKLARDLKAVKYVECSA LTQKGLKNVFDEAILAALEP PEPKKPKK

Data sets:
Data typeCount
13C chemical shifts710
15N chemical shifts154
1H chemical shifts1162

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Cdc421
2GDP2

Entities:

Entity 1, Cdc42 188 residues - Formula weight is not available

AMPylation at Y32

1   HISMETGLNTHRILELYSCYSVALVALVAL
2   GLYASPGLYALAVALGLYLYSTHRCYSLEU
3   LEUILESERTYRTHRTHRASNLYSPHEPRO
4   SERGLUTYRVALPROTHRVALPHEASPASN
5   TYRALAVALTHRVALMETILEGLYGLYGLU
6   PROTYRTHRLEUGLYLEUPHEASPTHRALA
7   GLYLEUGLUASPTYRASPARGLEUARGPRO
8   LEUSERTYRPROGLNTHRASPVALPHELEU
9   VALCYSPHESERVALVALSERPROSERSER
10   PHEGLUASNVALLYSGLULYSTRPVALPRO
11   GLUILETHRHISHISCYSPROLYSTHRPRO
12   PHELEULEUVALGLYTHRGLNILEASPLEU
13   ARGASPASPPROSERTHRILEGLULYSLEU
14   ALALYSASNLYSGLNLYSPROILETHRPRO
15   GLUTHRALAGLULYSLEUALAARGASPLEU
16   LYSALAVALLYSTYRVALGLUCYSSERALA
17   LEUTHRGLNLYSGLYLEULYSASNVALPHE
18   ASPGLUALAILELEUALAALALEUGLUPRO
19   PROGLUPROLYSLYSPROLYSLYS

Entity 2, GDP - C10 H15 N5 O11 P2 - 443.201 Da.

1   GDP

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SPARKY: Backbone or all simulated peaks