BMRB Entry 50169

Name: DnaJB1 J-domain
Published: 2020-11-11

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50169

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

DnaJB1 J-domain

Deposition date:

2020-01-26

Original release date:

2020-11-11

Authors:

Rosenzweig, Rina; Abayev-Avraham, Meital

Citation:

Citation: Faust, Ofrah; Abayev-Avraham, Meital; Wentink, Anne; Maurer, Michael; Nillegoda, Nadinath; London, Nir; Bukau, Bernd; Rosenzweig, Rina. "Hsp40s employ class-specific regulation to drive Hsp70 functional diversity" Nature 587, 489-494 (2020).
PubMed: 33177718

Assembly members:

Assembly members:
J-domain of DnaJB1, polymer, 71 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pPET29b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
J-domain of DnaJB1: GKDYYQTLGLARGASDEEIK RAYRRQALRYHPDKNKEPGA EEKFKEIAEAYDVLSDPRKR EIFDRYGEEGL

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1_B1JD_BMRB

Data type	Count
15N chemical shifts	66
1H chemical shifts	66

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	DnaJB1 J-domain	1

Entities:

Entity 1, DnaJB1 J-domain 71 residues - Formula weight is not available

1

GLY

LYS

ASP

TYR

GLN

THR

LEU

GLY

LEU

2

ALA

ARG

GLY

ALA

SER

ASP

GLU

ILE

LYS

3

ARG

ALA

TYR

ARG

GLN

ALA

LEU

ARG

TYR

4

HIS

PRO

ASP

LYS

ASN

LYS

GLU

PRO

GLY

ALA

5

GLU

LYS

PHE

LYS

GLU

ILE

ALA

GLU

ALA

6

TYR

ASP

VAL

LEU

SER

ASP

PRO

ARG

LYS

ARG

7

GLU

ILE

PHE

ASP

ARG

TYR

GLY

GLU

GLY

8

LEU

Samples:

sample_1: DnaJB1, [U-15N; U-13C], 2 mM; HEPES 50 mM; potassium chloride 50 mM; sodium azide 0.03%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 50 mM; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCACO	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v4.0.6, Bruker Biospin - collection, processing

CcpNMR v2.4.2, CCPN - data analysis

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks