BMRB Entry 50168

Name: J-domain region of DnaJA2
Published: 2020-11-11

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50168

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

J-domain region of DnaJA2

Deposition date:

2020-01-26

Original release date:

2020-11-11

Authors:

Rosenzweig, Rina; Abayev-Avraham, Meital

Citation:

Citation: Rosenzweig, Rina; Abayev-Avraham, Maital; Faust, Ofrah; Maurer, Michael; Wentink, Anne; Bukau, Bernd; Nillegoda, Nadinath; London, Nir. "Hsp40s employ class-specific regulation to drive Hsp70 functional diversity" Nature 587, 489-494 (2020).
PubMed: 33177718

Assembly members:

Assembly members:
J-domain of DnaJA2, polymer, 77 residues, 8777 Da.

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET29b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
J-domain of DnaJA2: MANVADTKLYDILGVPPGAS ENELKKAYRKLAKEYHPDKN PNAGDKFKEISFAYEVLSNP EKRELYDRYGEQGLREG

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1_A2JD

Data type	Count
13C chemical shifts	204
15N chemical shifts	69
1H chemical shifts	69

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	J-domain region of DnaJA2	1

Entities:

Entity 1, J-domain region of DnaJA2 77 residues - 8777 Da.

1

MET

ALA

ASN

VAL

ALA

ASP

THR

LYS

LEU

TYR

2

ASP

ILE

LEU

GLY

VAL

PRO

GLY

ALA

SER

3

GLU

ASN

GLU

LEU

LYS

ALA

TYR

ARG

LYS

4

LEU

ALA

LYS

GLU

TYR

HIS

PRO

ASP

LYS

ASN

5

PRO

ASN

ALA

GLY

ASP

LYS

PHE

LYS

GLU

ILE

6

SER

PHE

ALA

TYR

GLU

VAL

LEU

SER

ASN

PRO

7

GLU

LYS

ARG

GLU

LEU

TYR

ASP

ARG

TYR

GLY

8

GLU

GLN

GLY

LEU

ARG

GLU

GLY

Samples:

sample_1: HEPES 50 mM; KCl 50 mM; sodium azide 0.03%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 50 mM; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HCACO	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v4.0.6, Bruker Biospin - collection, processing

CcpNMR v2.4.2, CCPN - data analysis

NMR spectrometers:

Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks