BMRB Entry 50167

Title:
DnaJB1 J-domain+G/F rich region
Deposition date:
2020-01-26
Original release date:
2020-11-11
Authors:
Rosenzweig, Rina; Abayev-Avraham, Meital
Citation:

Citation: Faust, Ofrah; Abayev-Avraham, Meital; Wentink, Anne; Maurer, Michael; Nillegoda, Nadinath; London, Nir; Bukau, Bernd; Rosenzweig, Rina. "Hsp40s employ class-specific regulation to drive Hsp70 functional diversity"  Nature 587, 489-494 (2020).
PubMed: 33177718

Assembly members:

Assembly members:
B1GF, polymer, 109 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pPET29b

Data sets:
Data typeCount
13C chemical shifts414
15N chemical shifts93
1H chemical shifts515

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DnaJB1 J-domain+G/F rich region1

Entities:

Entity 1, DnaJB1 J-domain+G/F rich region 109 residues - Formula weight is not available

1   GLYLYSASPTYRTYRGLNTHRLEUGLYLEU
2   ALAARGGLYALASERASPGLUGLUILELYS
3   ARGALATYRARGARGGLNALALEUARGTYR
4   HISPROASPLYSASNLYSGLUPROGLYALA
5   GLUGLULYSPHELYSGLUILEALAGLUALA
6   TYRASPVALLEUSERASPPROARGLYSARG
7   GLUILEPHEASPARGTYRGLYGLUGLUGLY
8   LEULYSGLYSERGLYPROSERGLYGLYSER
9   GLYGLYGLYALAASNGLYTHRSERPHESER
10   TYRTHRPHEHISGLYASPPROHISALAMET
11   PHEALAGLUPHEPHEGLYGLYARGASN

Samples:

sample_1: DnaJB1GF, [U-15N; U-13C], 4 mM; HEPES 50 mM; potassium chloride 50 mM; sodium azide 0.03%; D2O, [U-2H], 10%

sample_2: DnaJB1GF, [U-15N;U-13C], 4 mM; HEPES 50 mM; potassium chloride 50 mM; sodium azide 0.03%; D2O, [U-2H], 99.9%

sample_conditions_1: ionic strength: 50 mM; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_2isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
2D HMQC_Methylsample_2isotropicsample_conditions_1
2D (HB)CB(CGCD)HDsample_1isotropicsample_conditions_1
2D (HB)CB(CGCDCE)HEsample_1isotropicsample_conditions_1

Software:

TOPSPIN v4.0.6, Bruker Biospin - collection, processing

CcpNMR v2.4.2, CCPN - data analysis

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz
  • Bruker Avance 1000 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks