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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50167
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
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Citation: Faust, Ofrah; Abayev-Avraham, Meital; Wentink, Anne; Maurer, Michael; Nillegoda, Nadinath; London, Nir; Bukau, Bernd; Rosenzweig, Rina. "Hsp40s employ class-specific regulation to drive Hsp70 functional diversity" Nature 587, 489-494 (2020).
PubMed: 33177718
Assembly members:
B1GF, polymer, 109 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pPET29b
Entity Sequences (FASTA):
B1GF: GKDYYQTLGLARGASDEEIK
RAYRRQALRYHPDKNKEPGA
EEKFKEIAEAYDVLSDPRKR
EIFDRYGEEGLKGSGPSGGS
GGGANGTSFSYTFHGDPHAM
FAEFFGGRN
Data type | Count |
13C chemical shifts | 414 |
15N chemical shifts | 93 |
1H chemical shifts | 515 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | DnaJB1 J-domain+G/F rich region | 1 |
Entity 1, DnaJB1 J-domain+G/F rich region 109 residues - Formula weight is not available
1 | GLY | LYS | ASP | TYR | TYR | GLN | THR | LEU | GLY | LEU | ||||
2 | ALA | ARG | GLY | ALA | SER | ASP | GLU | GLU | ILE | LYS | ||||
3 | ARG | ALA | TYR | ARG | ARG | GLN | ALA | LEU | ARG | TYR | ||||
4 | HIS | PRO | ASP | LYS | ASN | LYS | GLU | PRO | GLY | ALA | ||||
5 | GLU | GLU | LYS | PHE | LYS | GLU | ILE | ALA | GLU | ALA | ||||
6 | TYR | ASP | VAL | LEU | SER | ASP | PRO | ARG | LYS | ARG | ||||
7 | GLU | ILE | PHE | ASP | ARG | TYR | GLY | GLU | GLU | GLY | ||||
8 | LEU | LYS | GLY | SER | GLY | PRO | SER | GLY | GLY | SER | ||||
9 | GLY | GLY | GLY | ALA | ASN | GLY | THR | SER | PHE | SER | ||||
10 | TYR | THR | PHE | HIS | GLY | ASP | PRO | HIS | ALA | MET | ||||
11 | PHE | ALA | GLU | PHE | PHE | GLY | GLY | ARG | ASN |
sample_1: DnaJB1GF, [U-15N; U-13C], 4 mM; HEPES 50 mM; potassium chloride 50 mM; sodium azide 0.03%; D2O, [U-2H], 10%
sample_2: DnaJB1GF, [U-15N;U-13C], 4 mM; HEPES 50 mM; potassium chloride 50 mM; sodium azide 0.03%; D2O, [U-2H], 99.9%
sample_conditions_1: ionic strength: 50 mM; pH: 7.5; pressure: 1 atm; temperature: 298 K
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HCACO | sample_1 | isotropic | sample_conditions_1 |
3D HCCH-TOCSY | sample_2 | isotropic | sample_conditions_1 |
3D 1H-13C NOESY aliphatic | sample_2 | isotropic | sample_conditions_1 |
3D 1H-13C NOESY aromatic | sample_2 | isotropic | sample_conditions_1 |
3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
2D HMQC_Methyl | sample_2 | isotropic | sample_conditions_1 |
2D (HB)CB(CGCD)HD | sample_1 | isotropic | sample_conditions_1 |
2D (HB)CB(CGCDCE)HE | sample_1 | isotropic | sample_conditions_1 |
TOPSPIN v4.0.6, Bruker Biospin - collection, processing
CcpNMR v2.4.2, CCPN - data analysis
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