BMRB Entry 50163

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50163

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Title:
hyen E
Deposition date:
2020-01-22
Original release date:
2020-05-13
Authors:
Du, Qingdan
Citation:

Citation: Du, Qingdan; Chan, Lai; Gilding, Edward; Henriques, Sonia Troeira; Condon, Nicholas; Ravipati, Anjaneya; Kaas, Quentin; Huang, Yen-Hua; Craik, David. "Discovery and mechanistic studies of cytotoxic cyclotides from the medicinal herb Hybanthus enneaspermus"  J. Biol. Chem. 295, 10911-10925 (2020).
PubMed: 32414842

Assembly members:

Assembly members:
entity_1_hyen_E, polymer, 30 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Hybanthus enneaspermus   Taxonomy ID: 212266   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Hybanthus enneaspermus

Experimental source:

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1_hyen_E: GVPCGESCVYIPCFTGIINC SCRDKVCYNN

Data sets:
Data typeCount
1H chemical shifts61

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hyen E1

Entities:

Entity 1, hyen E 30 residues - Formula weight is not available

1   GLYVALPROCYSGLYGLUSERCYSVALTYR
2   ILEPROCYSPHETHRGLYILEILEASNCYS
3   SERCYSARGASPLYSVALCYSTYRASNASN

Samples:

sample_1_hyen_E: entity_1 1 mM

sample_conditions_1_hyen_E: ionic strength: 0 M; pH: 3.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1_hyen_Eisotropicsample_conditions_1_hyen_E
2D 1H-1H NOESYsample_1_hyen_Eisotropicsample_conditions_1_hyen_E

Software:

CcpNMR, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz