BMRB Entry 50156

Name: Backbone assignment of DUSP22
Published: 2021-08-12

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50156

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignment of DUSP22

Deposition date:

2020-01-13

Original release date:

2021-08-12

Authors:

Lai, Chih_Hsuan; Chang, Co-Chih; Lyu, Ping-Chiang

Citation:

Citation: Lai, Chih-Hsuan; Chang, Co-Chih; Chuang, Huai-Chia; Tan, Tse-Hua; Lyu, Ping-Chiang. "Structural Insights into the Active Site Formation of DUSP22 in N-loop-containing Protein Tyrosine Phosphatases" Intern. J. Mol. Sci. 21, 7515-7515 (2020).
PubMed: 33053837

Assembly members:

Assembly members:
DUSP22, polymer, 157 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DUSP22: GPMGNGMNKILPGLYIGNFK DARDAEQLSKNKVTHILSVH DSARPMLEGVKYLCIPAADS PSQNLTRHFKESIKFIHECR LRGESCLVHCLAGVSRSVTL VIAYIMTVTDFGWEDALHTV RAGRSCANPNVGFQRQLQEF EKHEVHQYRQWLKEEYG

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	405
15N chemical shifts	139
1H chemical shifts	139

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	DUSP22	1

Entities:

Entity 1, DUSP22 157 residues - Formula weight is not available

1

GLY

PRO

MET

GLY

ASN

GLY

MET

ASN

LYS

ILE

2

LEU

PRO

GLY

LEU

TYR

ILE

GLY

ASN

PHE

LYS

3

ASP

ALA

ARG

ASP

ALA

GLU

GLN

LEU

SER

LYS

4

ASN

LYS

VAL

THR

HIS

ILE

LEU

SER

VAL

HIS

5

ASP

SER

ALA

ARG

PRO

MET

LEU

GLU

GLY

VAL

6

LYS

TYR

LEU

CYS

ILE

PRO

ALA

ASP

SER

7

PRO

SER

GLN

ASN

LEU

THR

ARG

HIS

PHE

LYS

8

GLU

SER

ILE

LYS

PHE

ILE

HIS

GLU

CYS

ARG

9

LEU

ARG

GLY

GLU

SER

CYS

LEU

VAL

HIS

CYS

10

LEU

ALA

GLY

VAL

SER

ARG

SER

VAL

THR

LEU

11

VAL

ILE

ALA

TYR

ILE

MET

THR

VAL

THR

ASP

12

PHE

GLY

TRP

GLU

ASP

ALA

LEU

HIS

THR

VAL

13

ARG

ALA

GLY

ARG

SER

CYS

ALA

ASN

PRO

ASN

14

VAL

GLY

PHE

GLN

ARG

GLN

LEU

GLN

GLU

PHE

15

GLU

LYS

HIS

GLU

VAL

HIS

GLN

TYR

ARG

GLN

16

TRP

LEU

LYS

GLU

TYR

GLY

Samples:

sample_1_13C_15N_DUSP22: DUSP22, [13C, 15N], 0.3-1.0 mM; PIPES 40 mM; sodium chloride 100 mM; DSS 0.16 mM

sample_conditions_1_RT: pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1_13C_15N_DUSP22	isotropic	sample_conditions_1_RT
3D HN(COCA)CB	sample_1_13C_15N_DUSP22	isotropic	sample_conditions_1_RT
3D HNCACB	sample_1_13C_15N_DUSP22	isotropic	sample_conditions_1_RT
3D HNCA	sample_1_13C_15N_DUSP22	isotropic	sample_conditions_1_RT
3D HN(CO)CA	sample_1_13C_15N_DUSP22	isotropic	sample_conditions_1_RT
3D HNCO	sample_1_13C_15N_DUSP22	isotropic	sample_conditions_1_RT

Software:

TOPSPIN, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Bruker AMX 850 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks