BMRB Entry 50156

Title:
Backbone assignment of DUSP22
Deposition date:
2020-01-13
Original release date:
2021-08-12
Authors:
Lai, Chih_Hsuan; Chang, Co-Chih; Lyu, Ping-Chiang
Citation:

Citation: Lai, Chih-Hsuan; Chang, Co-Chih; Chuang, Huai-Chia; Tan, Tse-Hua; Lyu, Ping-Chiang. "Structural Insights into the Active Site Formation of DUSP22 in N-loop-containing Protein Tyrosine Phosphatases"  Intern. J. Mol. Sci. 21, 7515-7515 (2020).
PubMed: 33053837

Assembly members:

Assembly members:
DUSP22, polymer, 157 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21b

Data sets:
Data typeCount
13C chemical shifts405
15N chemical shifts139
1H chemical shifts139

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DUSP221

Entities:

Entity 1, DUSP22 157 residues - Formula weight is not available

1   GLYPROMETGLYASNGLYMETASNLYSILE
2   LEUPROGLYLEUTYRILEGLYASNPHELYS
3   ASPALAARGASPALAGLUGLNLEUSERLYS
4   ASNLYSVALTHRHISILELEUSERVALHIS
5   ASPSERALAARGPROMETLEUGLUGLYVAL
6   LYSTYRLEUCYSILEPROALAALAASPSER
7   PROSERGLNASNLEUTHRARGHISPHELYS
8   GLUSERILELYSPHEILEHISGLUCYSARG
9   LEUARGGLYGLUSERCYSLEUVALHISCYS
10   LEUALAGLYVALSERARGSERVALTHRLEU
11   VALILEALATYRILEMETTHRVALTHRASP
12   PHEGLYTRPGLUASPALALEUHISTHRVAL
13   ARGALAGLYARGSERCYSALAASNPROASN
14   VALGLYPHEGLNARGGLNLEUGLNGLUPHE
15   GLULYSHISGLUVALHISGLNTYRARGGLN
16   TRPLEULYSGLUGLUTYRGLY

Samples:

sample_1_13C_15N_DUSP22: DUSP22, [13C, 15N], 0.3-1.0 mM; PIPES 40 mM; sodium chloride 100 mM; DSS 0.16 mM

sample_conditions_1_RT: pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1_13C_15N_DUSP22isotropicsample_conditions_1_RT
3D HN(COCA)CBsample_1_13C_15N_DUSP22isotropicsample_conditions_1_RT
3D HNCACBsample_1_13C_15N_DUSP22isotropicsample_conditions_1_RT
3D HNCAsample_1_13C_15N_DUSP22isotropicsample_conditions_1_RT
3D HN(CO)CAsample_1_13C_15N_DUSP22isotropicsample_conditions_1_RT
3D HNCOsample_1_13C_15N_DUSP22isotropicsample_conditions_1_RT

Software:

TOPSPIN, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker AMX 850 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks