BMRB Entry 50154

Name: C-terminal domain of TDP-43 at acidic pH
Published: 2021-03-10

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50154

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

C-terminal domain of TDP-43 at acidic pH

Deposition date:

2020-01-09

Original release date:

2021-03-10

Authors:

Mompean, Miguel

Citation:

Citation: Pantoja-Uceda, David; Stuani, Cristiana; Laurents, Douglas; McDermott, Ann; Buratti, Emanuele; Mompean, Miguel. "NMR assignments for the C-terminal domain of human TDP-43" Biomol. NMR Assign. 15, 177-181 (2021).
PubMed: 33417141

Assembly members:

Assembly members:
TDP-43 267-414, polymer, 169 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pJ411

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TDP-43 267-414: MGSDKIHHHHHHENLYFQGH MNRQLERSGRFGGNPGGFGN QGGFGNSRGGGAGLGNNQGS NMGGGMNFGAFSINPAMMAA AQAALQSSWGMMGMLASQQN QSGPSGNNQNQGNMQREPNQ AFGSGNNSYSGSNSGAAIGW GSASNAGSGSGFNGGFGSSM DSKSSGWGM

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	467
15N chemical shifts	163
1H chemical shifts	552

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	C-terminal domain of TDP-43	1

Entities:

Entity 1, C-terminal domain of TDP-43 169 residues - Formula weight is not available

1

MET

GLY

SER

ASP

LYS

ILE

HIS

2

HIS

GLU

ASN

LEU

TYR

PHE

GLN

GLY

HIS

3

MET

ASN

ARG

GLN

LEU

GLU

ARG

SER

GLY

ARG

4

PHE

GLY

ASN

PRO

GLY

PHE

GLY

ASN

5

GLN

GLY

PHE

GLY

ASN

SER

ARG

GLY

6

GLY

ALA

GLY

LEU

GLY

ASN

GLN

GLY

SER

7

ASN

MET

GLY

MET

ASN

PHE

GLY

ALA

8

PHE

SER

ILE

ASN

PRO

ALA

MET

ALA

9

ALA

GLN

ALA

LEU

GLN

SER

TRP

GLY

10

MET

GLY

MET

LEU

ALA

SER

GLN

ASN

11

GLN

SER

GLY

PRO

SER

GLY

ASN

GLN

ASN

12

GLN

GLY

ASN

MET

GLN

ARG

GLU

PRO

ASN

GLN

13

ALA

PHE

GLY

SER

GLY

ASN

SER

TYR

SER

14

GLY

SER

ASN

SER

GLY

ALA

ILE

GLY

TRP

15

GLY

SER

ALA

SER

ASN

ALA

GLY

SER

GLY

SER

16

GLY

PHE

ASN

GLY

PHE

GLY

SER

MET

17

ASP

SER

LYS

SER

GLY

TRP

GLY

MET

Samples:

sample_1: entity_1, [U-100% 13C; U-100% 15N], 60-120 uM

sample_conditions_1: pH: 4; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D CC(CO)NH	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(NCOCA)NH	sample_1	isotropic	sample_conditions_1
3D (H)N(COCA)NH	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR, CCPN - chemical shift assignment

NMR spectrometers:

Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks