BMRB Entry 50151

Name: Partial backbone resonance assignments for Human DND1-RRM2
Published: 2021-08-12

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50151

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Partial backbone resonance assignments for Human DND1-RRM2

Deposition date:

2020-01-07

Original release date:

2021-08-12

Authors:

Kumari, Pooja

Citation:

Citation: Kumari, Pooja; Bhavesh, Neel Sarovar. "Human DND1-RRM2 forms a non-canonical domain swapped dimer" Protein Sci. 30, 1184-1195 (2021).
PubMed: 33860980

Assembly members:

Assembly members:
DND1-RRM2, polymer, 100 residues, 10553.19 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DND1-RRM2: MGSSHHHHHHSSGLVPRGSH MELSVDGLPPNLTRSALLLA LQPLGPGLQEARLLPSPGPA PGQIALLKFSSHRAAAMAKK ALVEGQSHLCGEQVAVEWLK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1_DND1-RRM2

Data type	Count
13C chemical shifts	190
15N chemical shifts	67
1H chemical shifts	67

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	DND1-RRM2	1

Entities:

Entity 1, DND1-RRM2 100 residues - 10553.19 Da.

Residue 1-21 belongs to hexa-histidine tag

1	MET	GLY	SER	SER	HIS	HIS	HIS	HIS	HIS	HIS
2	SER	SER	GLY	LEU	VAL	PRO	ARG	GLY	SER	HIS
3	MET	GLU	LEU	SER	VAL	ASP	GLY	LEU	PRO	PRO
4	ASN	LEU	THR	ARG	SER	ALA	LEU	LEU	LEU	ALA
5	LEU	GLN	PRO	LEU	GLY	PRO	GLY	LEU	GLN	GLU
6	ALA	ARG	LEU	LEU	PRO	SER	PRO	GLY	PRO	ALA
7	PRO	GLY	GLN	ILE	ALA	LEU	LEU	LYS	PHE	SER
8	SER	HIS	ARG	ALA	ALA	ALA	MET	ALA	LYS	LYS
9	ALA	LEU	VAL	GLU	GLY	GLN	SER	HIS	LEU	CYS
10	GLY	GLU	GLN	VAL	ALA	VAL	GLU	TRP	LEU	LYS

Samples:

sample_1_RRM2: DND1-RRM2, [U-100% 13C; U-100% 15N], 0.5 mM; sodium phosphate 25 mM; sodium chloride 100 mM

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1_RRM2	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1_RRM2	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1_RRM2	isotropic	sample_conditions_1
3D HNCO	sample_1_RRM2	isotropic	sample_conditions_1
3D HNCACB	sample_1_RRM2	isotropic	sample_conditions_1
3D HNCA	sample_1_RRM2	isotropic	sample_conditions_1

Software:

TOPSPIN v3.6.0, Bruker Biospin - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

Bruker Avance 500 MHz

1	MET	GLY	SER	SER	HIS	HIS	HIS	HIS	HIS	HIS
2	SER	SER	GLY	LEU	VAL	PRO	ARG	GLY	SER	HIS
3	MET	GLU	LEU	SER	VAL	ASP	GLY	LEU	PRO	PRO
4	ASN	LEU	THR	ARG	SER	ALA	LEU	LEU	LEU	ALA
5	LEU	GLN	PRO	LEU	GLY	PRO	GLY	LEU	GLN	GLU
6	ALA	ARG	LEU	LEU	PRO	SER	PRO	GLY	PRO	ALA
7	PRO	GLY	GLN	ILE	ALA	LEU	LEU	LYS	PHE	SER
8	SER	HIS	ARG	ALA	ALA	ALA	MET	ALA	LYS	LYS
9	ALA	LEU	VAL	GLU	GLY	GLN	SER	HIS	LEU	CYS
10	GLY	GLU	GLN	VAL	ALA	VAL	GLU	TRP	LEU	LYS

1	MET	GLY	SER	SER	HIS	HIS	HIS	HIS	HIS	HIS
2	SER	SER	GLY	LEU	VAL	PRO	ARG	GLY	SER	HIS
3	MET	GLU	LEU	SER	VAL	ASP	GLY	LEU	PRO	PRO
4	ASN	LEU	THR	ARG	SER	ALA	LEU	LEU	LEU	ALA
5	LEU	GLN	PRO	LEU	GLY	PRO	GLY	LEU	GLN	GLU
6	ALA	ARG	LEU	LEU	PRO	SER	PRO	GLY	PRO	ALA
7	PRO	GLY	GLN	ILE	ALA	LEU	LEU	LYS	PHE	SER
8	SER	HIS	ARG	ALA	ALA	ALA	MET	ALA	LYS	LYS
9	ALA	LEU	VAL	GLU	GLY	GLN	SER	HIS	LEU	CYS
10	GLY	GLU	GLN	VAL	ALA	VAL	GLU	TRP	LEU	LYS