BMRB Entry 50148

Name: 1H, 15N, and 13C backbone chemical shift assignments for the first bromodomain of BRD3 (BRD3-BD1)
Published: 2021-08-12

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50148

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N, and 13C backbone chemical shift assignments for the first bromodomain of BRD3 (BRD3-BD1)

Deposition date:

2019-12-31

Original release date:

2021-08-12

Authors:

Patel, Karishma; Mackay, Joel; Solomon, Paul

Citation:

Citation: Patel, Karishma; Solomon, Paul; Walshe, James; Ford, Daniel; Wilkinson-White, Lorna; Payne, Richard; Low, Jason; Mackay, Joel. "BET-Family Bromodomains Can Recognize Diacetylated Sequences from Transcription Factors Using a Conserved Mechanism" Biochemistry 60, 648-662 (2021).
PubMed: 33620209

Assembly members:

Assembly members:
The first bromodomain of BRD3, polymer, 128 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-6P

Entity Sequences (FASTA):

Entity Sequences (FASTA):
The first bromodomain of BRD3: QPLGSEVSNPSKPGRKTNQL QYMQNVVVKTLWKHQFAWPF YQPVDAIKLNLPDYHKIIKN PMDMGTIKKRLENNYYWSAS ECMQDFNTMFTNCYIYNKPT DDIVLMAQALEKIFLQKVAQ MPQEEVEL

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	244
15N chemical shifts	112
1H chemical shifts	112

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	The first bromodomain of BRD3	1

Entities:

Entity 1, The first bromodomain of BRD3 128 residues - Formula weight is not available

1

GLN

PRO

LEU

GLY

SER

GLU

VAL

SER

ASN

PRO

2

SER

LYS

PRO

GLY

ARG

LYS

THR

ASN

GLN

LEU

3

GLN

TYR

MET

GLN

ASN

VAL

LYS

THR

4

LEU

TRP

LYS

HIS

GLN

PHE

ALA

TRP

PRO

PHE

5

TYR

GLN

PRO

VAL

ASP

ALA

ILE

LYS

LEU

ASN

6

LEU

PRO

ASP

TYR

HIS

LYS

ILE

LYS

ASN

7

PRO

MET

ASP

MET

GLY

THR

ILE

LYS

ARG

8

LEU

GLU

ASN

TYR

TRP

SER

ALA

SER

9

GLU

CYS

MET

GLN

ASP

PHE

ASN

THR

MET

PHE

10

THR

ASN

CYS

TYR

ILE

TYR

ASN

LYS

PRO

THR

11

ASP

ILE

VAL

LEU

MET

ALA

GLN

ALA

LEU

12

GLU

LYS

ILE

PHE

LEU

GLN

LYS

VAL

ALA

GLN

13

MET

PRO

GLN

GLU

VAL

GLU

LEU

Samples:

sample_1: DSS 0.01 mM; TRIS 10 mM; sodium chloride 100 mM; DTT 1 mM

sample_conditions_1: ionic strength: 100 mM; pH: 7.2; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR, CCPN - chemical shift assignment

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks