BMRB Entry 50146

Title:
1H, 15N, and 13C backbone chemical shift assignments for the second bromodomain of BRD4 (BRD4-BD2)
Deposition date:
2019-12-31
Original release date:
2021-08-12
Authors:
Patel, Karishma; Mackay, Joel; Solomon, Paul
Citation:

Citation: Patel, Karishma; Solomon, Paul; Walshe, James; Ford, Daniel; Wilkinson-White, Lorna; Payne, Richard; Low, Jason; Mackay, Joel. "BET-Family Bromodomains Can Recognize Diacetylated Sequences from Transcription Factors Using a Conserved Mechanism"  Biochemistry 60, 648-662 (2021).
PubMed: 33620209

Assembly members:

Assembly members:
The second bromdomain of BRD4, polymer, 122 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-6P

Data sets:
Data typeCount
13C chemical shifts143
15N chemical shifts84
1H chemical shifts84

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1The second bromodomain of BRD41

Entities:

Entity 1, The second bromodomain of BRD4 122 residues - Formula weight is not available

1   GLYPROLEUGLYSERSERLYSVALSERGLU
2   GLNLEULYSCYSCYSSERGLYILELEULYS
3   GLUMETPHEALALYSLYSHISALAALATYR
4   ALATRPPROPHETYRLYSPROVALASPVAL
5   GLUALALEUGLYLEUHISASPTYRCYSASP
6   ILEILELYSHISPROMETASPMETSERTHR
7   ILELYSSERLYSLEUGLUALAARGGLUTYR
8   ARGASPALAGLNGLUPHEGLYALAASPVAL
9   ARGLEUMETPHESERASNCYSTYRLYSTYR
10   ASNPROPROASPHISGLUVALVALALAMET
11   ALAARGLYSLEUGLNASPVALPHEGLUMET
12   ARGPHEALALYSMETPROASPGLUPROGLU
13   GLUPRO

Samples:

sample_1: DSS 0.01 mM; TRIS 10 mM; sodium chloride 100 mM; DTT 1 mM

sample_conditions_1: ionic strength: 100 mM; pH: 7.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

CcpNMR, CCPN - chemical shift assignment

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks