BMRB Entry 5013

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5013

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N and 13C Assignments of the Catalytic Domain of E6-associated Protein (E6AP)

Deposition date:

2001-05-18

Original release date:

2002-01-23

Authors:

Wu, Yu-Sung; Domaille, Peter

Citation:

Citation: Wu, Yu-Sung; Domaille, Peter. "1H, 15N and 13C Assignments of the Catalytic Domain of E6-associated Protein (E6AP)" J. Biomol. NMR 21, 285-286 (2001).
PubMed: 11775746

Assembly members:

Assembly members:
E6-associated protein, polymer, 120 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Vector: pGEX-4T

Entity Sequences (FASTA):

Entity Sequences (FASTA):
E6-associated protein: ELLICGSRNLDFQALEETTE YDGGYTRDSVLIREFWEIVH SFTDEQKRLFLQFTTGTDRA PVGGLGKLKMIIAKNGPDTE RLPTSHTCFNVLLLPEYSSK EKLKERLLKAITYAKGFGML

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
1H chemical shifts	747
13C chemical shifts	492
15N chemical shifts	118

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	E6AP catalytic domain	1

Entities:

Entity 1, E6AP catalytic domain 120 residues - Formula weight is not available

1	GLU	LEU	LEU	ILE	CYS	GLY	SER	ARG	ASN	LEU
2	ASP	PHE	GLN	ALA	LEU	GLU	GLU	THR	THR	GLU
3	TYR	ASP	GLY	GLY	TYR	THR	ARG	ASP	SER	VAL
4	LEU	ILE	ARG	GLU	PHE	TRP	GLU	ILE	VAL	HIS
5	SER	PHE	THR	ASP	GLU	GLN	LYS	ARG	LEU	PHE
6	LEU	GLN	PHE	THR	THR	GLY	THR	ASP	ARG	ALA
7	PRO	VAL	GLY	GLY	LEU	GLY	LYS	LEU	LYS	MET
8	ILE	ILE	ALA	LYS	ASN	GLY	PRO	ASP	THR	GLU
9	ARG	LEU	PRO	THR	SER	HIS	THR	CYS	PHE	ASN
10	VAL	LEU	LEU	LEU	PRO	GLU	TYR	SER	SER	LYS
11	GLU	LYS	LEU	LYS	GLU	ARG	LEU	LEU	LYS	ALA
12	ILE	THR	TYR	ALA	LYS	GLY	PHE	GLY	MET	LEU

Samples:

sample_1: E6-associated protein, [U-15N; U-13C], 0.9 mM

cond_1: pH: 7.5; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N HSQC	not available	not available	not available
HNCACB	not available	not available	not available
CBCACONH	not available	not available	not available
HNCO	not available	not available	not available
HBCB/HACACONH	not available	not available	not available
HCCH-TOCSY	not available	not available	not available

Software:

ANSIG v3.3 - peak picking, peak assignments

NMR spectrometers:

Bruker Avance 600 MHz

PDB	1C4Z 1D5F
DBJ	BAD69554 BAD90321 BAE02425 BAE37676 BAF84094
EMBL	CAA66653 CAA66654 CAA66655 CAA66656 CAH92897
GB	AAA35542 AAB47756 AAB49301 AAB69154 AAC83345
PIR	A38920
REF	NP_000453 NP_001029134 NP_001091932 NP_001126699 NP_001178766
SP	Q05086
TPG	DAA17742
AlphaFold	Q05086

1	GLU	LEU	LEU	ILE	CYS	GLY	SER	ARG	ASN	LEU
2	ASP	PHE	GLN	ALA	LEU	GLU	GLU	THR	THR	GLU
3	TYR	ASP	GLY	GLY	TYR	THR	ARG	ASP	SER	VAL
4	LEU	ILE	ARG	GLU	PHE	TRP	GLU	ILE	VAL	HIS
5	SER	PHE	THR	ASP	GLU	GLN	LYS	ARG	LEU	PHE
6	LEU	GLN	PHE	THR	THR	GLY	THR	ASP	ARG	ALA
7	PRO	VAL	GLY	GLY	LEU	GLY	LYS	LEU	LYS	MET
8	ILE	ILE	ALA	LYS	ASN	GLY	PRO	ASP	THR	GLU
9	ARG	LEU	PRO	THR	SER	HIS	THR	CYS	PHE	ASN
10	VAL	LEU	LEU	LEU	PRO	GLU	TYR	SER	SER	LYS
11	GLU	LYS	LEU	LYS	GLU	ARG	LEU	LEU	LYS	ALA
12	ILE	THR	TYR	ALA	LYS	GLY	PHE	GLY	MET	LEU

1	GLU	LEU	LEU	ILE	CYS	GLY	SER	ARG	ASN	LEU
2	ASP	PHE	GLN	ALA	LEU	GLU	GLU	THR	THR	GLU
3	TYR	ASP	GLY	GLY	TYR	THR	ARG	ASP	SER	VAL
4	LEU	ILE	ARG	GLU	PHE	TRP	GLU	ILE	VAL	HIS
5	SER	PHE	THR	ASP	GLU	GLN	LYS	ARG	LEU	PHE
6	LEU	GLN	PHE	THR	THR	GLY	THR	ASP	ARG	ALA
7	PRO	VAL	GLY	GLY	LEU	GLY	LYS	LEU	LYS	MET
8	ILE	ILE	ALA	LYS	ASN	GLY	PRO	ASP	THR	GLU
9	ARG	LEU	PRO	THR	SER	HIS	THR	CYS	PHE	ASN
10	VAL	LEU	LEU	LEU	PRO	GLU	TYR	SER	SER	LYS
11	GLU	LYS	LEU	LYS	GLU	ARG	LEU	LEU	LYS	ALA
12	ILE	THR	TYR	ALA	LYS	GLY	PHE	GLY	MET	LEU