BMRB Entry 50110
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR50110
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Citation: Chagot, Marie-Eve; Quinternet, Marc; Jacquemin, Clemence; Manival, Xavier; Gardiennet, Carole. "Box C/D snoRNPs: Solid-State NMR Fingerprint of an Early-Stage 50 kDa Assembly Intermediate" Biomol. NMR Assignments 14, 131-140 (2020).
PubMed: 32030621
Assembly members:
entity_1, polymer, 129 residues, 13568.90 Da.
entity_2, polymer, 118 residues, 14072.15 Da.
entity_3, polymer, 95 residues, Formula weight is not available
Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pnEA
Entity Sequences (FASTA):
entity_1: GPHMSAPNPKAFPLADAALT
QQILDVVQQAANLRQLKKGA
NEATKTLNRGISEFIIMAAD
CEPIEILLHLPLLCEDKNVP
YVFVPSRVALGRACGVSRPV
IAASITTNDASAIKTQIYAV
KDKIETLLI
entity_2: GPHMDEDVKKWREERKKMWL
LKISNNKQKHMQEMGIKEDE
LKSQPSIFKESRKEKQFIQS
IQNQVQRGNPKIDLNLKLIQ
REFANENSQLLDFIRELGDV
GLLEYELSQQEKDVLFGS
entity_3: MNKTLKTKAFDDIYQNSAEL
QELLKYNTVKFHLAKVYRIL
SSTVNDGSSGKMNSDLQKEL
AVNYLNTLRYGGIHYNEAIE
EFCQILLDKLNAVKK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 410 |
| 15N chemical shifts | 106 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Snu13p | 1 |
| 2 | Rsa1p(238-352) | 2 |
| 3 | Hit1p(70-164) | 3 |
Entities:
Entity 1, Snu13p 129 residues - 13568.90 Da.
| 1 | GLY | PRO | HIS | MET | SER | ALA | PRO | ASN | PRO | LYS | ||||
| 2 | ALA | PHE | PRO | LEU | ALA | ASP | ALA | ALA | LEU | THR | ||||
| 3 | GLN | GLN | ILE | LEU | ASP | VAL | VAL | GLN | GLN | ALA | ||||
| 4 | ALA | ASN | LEU | ARG | GLN | LEU | LYS | LYS | GLY | ALA | ||||
| 5 | ASN | GLU | ALA | THR | LYS | THR | LEU | ASN | ARG | GLY | ||||
| 6 | ILE | SER | GLU | PHE | ILE | ILE | MET | ALA | ALA | ASP | ||||
| 7 | CYS | GLU | PRO | ILE | GLU | ILE | LEU | LEU | HIS | LEU | ||||
| 8 | PRO | LEU | LEU | CYS | GLU | ASP | LYS | ASN | VAL | PRO | ||||
| 9 | TYR | VAL | PHE | VAL | PRO | SER | ARG | VAL | ALA | LEU | ||||
| 10 | GLY | ARG | ALA | CYS | GLY | VAL | SER | ARG | PRO | VAL | ||||
| 11 | ILE | ALA | ALA | SER | ILE | THR | THR | ASN | ASP | ALA | ||||
| 12 | SER | ALA | ILE | LYS | THR | GLN | ILE | TYR | ALA | VAL | ||||
| 13 | LYS | ASP | LYS | ILE | GLU | THR | LEU | LEU | ILE |
Entity 2, Rsa1p(238-352) 118 residues - 14072.15 Da.
| 1 | GLY | PRO | HIS | MET | ASP | GLU | ASP | VAL | LYS | LYS | ||||
| 2 | TRP | ARG | GLU | GLU | ARG | LYS | LYS | MET | TRP | LEU | ||||
| 3 | LEU | LYS | ILE | SER | ASN | ASN | LYS | GLN | LYS | HIS | ||||
| 4 | MET | GLN | GLU | MET | GLY | ILE | LYS | GLU | ASP | GLU | ||||
| 5 | LEU | LYS | SER | GLN | PRO | SER | ILE | PHE | LYS | GLU | ||||
| 6 | SER | ARG | LYS | GLU | LYS | GLN | PHE | ILE | GLN | SER | ||||
| 7 | ILE | GLN | ASN | GLN | VAL | GLN | ARG | GLY | ASN | PRO | ||||
| 8 | LYS | ILE | ASP | LEU | ASN | LEU | LYS | LEU | ILE | GLN | ||||
| 9 | ARG | GLU | PHE | ALA | ASN | GLU | ASN | SER | GLN | LEU | ||||
| 10 | LEU | ASP | PHE | ILE | ARG | GLU | LEU | GLY | ASP | VAL | ||||
| 11 | GLY | LEU | LEU | GLU | TYR | GLU | LEU | SER | GLN | GLN | ||||
| 12 | GLU | LYS | ASP | VAL | LEU | PHE | GLY | SER |
Entity 3, Hit1p(70-164) 95 residues - Formula weight is not available
native
| 1 | MET | ASN | LYS | THR | LEU | LYS | THR | LYS | ALA | PHE | ||||
| 2 | ASP | ASP | ILE | TYR | GLN | ASN | SER | ALA | GLU | LEU | ||||
| 3 | GLN | GLU | LEU | LEU | LYS | TYR | ASN | THR | VAL | LYS | ||||
| 4 | PHE | HIS | LEU | ALA | LYS | VAL | TYR | ARG | ILE | LEU | ||||
| 5 | SER | SER | THR | VAL | ASN | ASP | GLY | SER | SER | GLY | ||||
| 6 | LYS | MET | ASN | SER | ASP | LEU | GLN | LYS | GLU | LEU | ||||
| 7 | ALA | VAL | ASN | TYR | LEU | ASN | THR | LEU | ARG | TYR | ||||
| 8 | GLY | GLY | ILE | HIS | TYR | ASN | GLU | ALA | ILE | GLU | ||||
| 9 | GLU | PHE | CYS | GLN | ILE | LEU | LEU | ASP | LYS | LEU | ||||
| 10 | ASN | ALA | VAL | LYS | LYS |
Samples:
sample_1: Snu13p, [U-100% 13C; U-100% 15N], 200 mg/mL; Rsa1p(238-352) 200 mg/mL; Hit1p(70-164) 200 mg/mL; HEPES 10 mM; sodium chloride 150 mM; TCEP 0.5 mM; sodium azide 0.05 % w/v
sample_conditions_1: ionic strength: 150 mM; pH: 7.5; pressure: 1 atm; temperature: 278 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D DARR | sample_1 | isotropic | sample_conditions_1 |
| 2D DARR | sample_1 | isotropic | sample_conditions_1 |
| 2D DREAM | sample_1 | isotropic | sample_conditions_1 |
| 2D NCA | sample_1 | isotropic | sample_conditions_1 |
| 2D NCO | sample_1 | isotropic | sample_conditions_1 |
| 3D NCACX | sample_1 | isotropic | sample_conditions_1 |
| 3D NCACX | sample_1 | isotropic | sample_conditions_1 |
| 3D NCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CANCO | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v3.5, Bruker Biospin - processing
CcpNMR v2.4, CCPN - chemical shift assignment, data analysis
NMR spectrometers:
- Bruker Avance 800 MHz
- Bruker Avance 950 MHz