BMRB Entry 50109

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50109

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Title:
Nonexchangeable proton shifts for d(GTATGGCCATAC)2
Deposition date:
2019-11-30
Original release date:
2019-12-17
Authors:
Lyon, Rhiannon; Southam, Hannah; Trevitt, Clare; Liao, Chunyan; El-Khamisy, Sherif; Poole, Robert
Citation:

Citation: Lyon, Rhiannon; Southam, Hannah; Trevitt, Clare; Liao, Chunyan; El-Khamisy, Sherif; Poole, Robert; Williamson, Mike. "CORM-3 induces DNA damage through Ru(II) binding to DNA"  Biochem. J. 479, 1429-1439 (2022).
PubMed: 35726678

Assembly members:

Assembly members:
entity_1, polymer, 12 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GTATGGCCATAC

Data sets:
Data typeCount
1H chemical shifts83

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1DNA strand, 11
2DNA strand, 21

Entities:

Entity 1, DNA strand, 1 12 residues - Formula weight is not available

1   DGDTDADTDGDGDCDCDADT
2   DADC

Samples:

sample_1: entity_1 5 mM; potassium phosphate 30 ± 1 mM

sample_conditions_1: pH: 7.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

TOPSPIN v2, Bruker Biospin - NMR processing and display

NMR spectrometers:

  • Bruker Avance 800 MHz