BMRB Entry 50095

Name: 1H, 15N chemical shift assignments of the imino groups of yeast tRNAPhe: influence of the post-transcriptional modifications
Published: 2020-09-19

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50095

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N chemical shift assignments of the imino groups of yeast tRNAPhe: influence of the post-transcriptional modifications

Deposition date:

2019-11-28

Original release date:

2020-09-19

Authors:

Catala, Marjorie; Gato, Alexandre; Tisne, Carine; Barraud, Pierre

Citation:

Citation: Catala, Marjorie; Gato, Alexandre; Tisne, Carine; Barraud, Pierre. "1H, 15N chemical shift assignments of the imino groups of yeast tRNA Phe: influence of the post-transcriptional modifications" Biomol. NMR Assignments 14, 169-174 (2020).
PubMed: 32239363

Assembly members:

Assembly members:
unmodified yeast tRNAPhe, polymer, 76 residues, Formula weight is not available

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: enzymatic semisynthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
unmodified yeast tRNAPhe: GCGGAUUUAGCUCAGUUGGG AGAGCGCCAGACUGAAGAUC UGGAGGUCCUGUGUUCGAUC CACAGAAUUCGCACCA

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
15N chemical shifts	27
1H chemical shifts	27

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	tRNAPhe	1

Entities:

Entity 1, tRNAPhe 76 residues - Formula weight is not available

1

G

C

G

A

U

A

G

2

C

U

C

A

G

U

G

3

A

G

A

G

C

G

C

A

G

4

A

C

U

G

A

G

A

U

C

5

U

G

A

G

U

C

U

6

G

U

G

U

C

G

A

U

C

7

C

A

C

A

G

A

U

C

8

G

C

A

C

A

Samples:

sample_1: entity_1 1.8-2.0 mM; sodium phosphate 10 mM; magnesium chloride 10 mM

sample_2: entity_1, [U-15N]-Gua, [U-15N]-Ura, 1.5-1.8 mM; sodium phosphate 10 mM; magnesium chloride 10 mM

sample_conditions_1: ionic strength: 20 mM; pH: 6.5; pressure: 1 atm; temperature: 311 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-15N BEST-TROSY	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_2	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_2	isotropic	sample_conditions_1

Software:

TOPSPIN v3.5, Bruker Biospin - collection, processing

SPARKY, Goddard - chemical shift assignment, data analysis

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 700 MHz