BMRB Entry 50093

Name: C-terminal lobe of NOX5 EF-domain calcium loaded
Published: 2020-01-10

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR50093

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

C-terminal lobe of NOX5 EF-domain calcium loaded

Deposition date:

2019-11-28

Original release date:

2020-01-10

Authors:

Pastore, Annalisa; Millana Fananas, Elisa

Citation:

Citation: Millana, Elisa; Todesca, Sofia; Sicorello, Alessandro; Masino, Laura; Pompach, Petr; Magnani, Francesca; Pastore, Annalisa; Mattevi, Andrea. "On the mechanism of calcium-dependent activation of NADPH oxidase 5 (NOX5)" FEBS J. 287, 2486-2503 (2020).
PubMed: 31785178

Assembly members:

Assembly members:
entity_1, polymer, . residues, 9715.906 Da.
entity_CA, non-polymer, 40.078 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: E. coli Vector: pET28 a+

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GDKLKFLFQVYDIDGSGSID PDELRTVLQSCLRESAISLP DEKLDQLTLALFESADADGN GAITFEELRDELQRFPGVME NLTISAAA

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	137
15N chemical shifts	66
1H chemical shifts	66

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	C-lobe of EF-domqin, hNOX5beta	1
2	Ca2+, 1	2
3	Ca2+, 2	2

Entities:

Entity 1, C-lobe of EF-domqin, hNOX5beta - 9715.906 Da.

The first amino acid (G) and the last three (AAA) where introduce due to cloning needs.

1

GLY

ASP

LYS

LEU

LYS

PHE

LEU

PHE

GLN

VAL

2

TYR

ASP

ILE

ASP

GLY

SER

GLY

SER

ILE

ASP

3

PRO

ASP

GLU

LEU

ARG

THR

VAL

LEU

GLN

SER

4

CYS

LEU

ARG

GLU

SER

ALA

ILE

SER

LEU

PRO

5

ASP

GLU

LYS

LEU

ASP

GLN

LEU

THR

LEU

ALA

6

LEU

PHE

GLU

SER

ALA

ASP

ALA

ASP

GLY

ASN

7

GLY

ALA

ILE

THR

PHE

GLU

LEU

ARG

ASP

8

GLU

LEU

GLN

ARG

PHE

PRO

GLY

VAL

MET

GLU

9

ASN

LEU

THR

ILE

SER

ALA

Entity 2, Ca2+, 1 - Ca - 40.078 Da.

1

CA

Samples:

sample_1: C-term lobe of hNOX5beta: residues 78-161, [U-100% 13C; U-100% 15N], 200 uM; HEPES pH 7 50 mM; NaCl 25 mM; D2O 5 % v/v; CaCl2 1 mM

sample_conditions_1: ionic strength: 25 mM; pH: 7; pressure: 1 atm; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
HSQC	sample_1	isotropic	sample_conditions_1
HNCACB	sample_1	isotropic	sample_conditions_1
HNcoCANH	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - Data collection

NMRPipe, Delaglio, Zhengrong and Bax - processing

NMRDraw, Delaglio, Zhengrong and Bax - processing

CcpNmr_Analysis, CCPN - chemical shift assignment and data analysis

NMR spectrometers:

Bruker Avance III 700, 800 and 950 MHz MHz

UNP	Q96PH1-4
AlphaFold	Q9HAM8