BMRB Entry 50082

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50082

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Title:
hGrb2
Deposition date:
2019-10-31
Original release date:
2022-08-11
Authors:
Pinet, Louise; Wang, Ying-Hui; Vogel, Anais; Guerlesquin, Francoise; Assrir, Nadine; van Heijenoort, Carine
Citation:

Citation: Pinet, Louise; Wang, Ying-Hui; Vogel, Anais; Guerlesquin, Francoise; Assrir, Nadine; van Heijenoort, Carine. "1H, 13C and 15N assignments of human Grb2 free of ligands"  Biomol. NMR Assign. 14, 323-327 (2020).
PubMed: 32844357

Assembly members:

Assembly members:
entity_1, polymer, 219 residues, 25334 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETM11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GAMEAIAKYDFKATADDELS FKRGDILKVLNEECDQNWYK AELNGKDGFIPKNYIEMKPH PWFFGKIPRAKAEEMLSKQR HDGAFLIRESESAPGDFSLS VKFGNDVQHFKVLRDGAGKY FLWVVKFNSLNELVDYHRST SVSRNQQIFLRDIEQVPQQP TYVQALFDFDPQEDGELGFR RGDFIHVMDNSDPNWWKGAC HGQTGMFPRNYVTPVNRNV

Data sets:
Data typeCount
13C chemical shifts555
15N chemical shifts173
1H chemical shifts173

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GRB21

Entities:

Entity 1, GRB2 219 residues - 25334 Da.

Sequence numbering is sequential, from -1,G to 217,V. The first two residues come from TEV cleavage site of the purification tag.

1   GLYALAMETGLUALAILEALALYSTYRASP
2   PHELYSALATHRALAASPASPGLULEUSER
3   PHELYSARGGLYASPILELEULYSVALLEU
4   ASNGLUGLUCYSASPGLNASNTRPTYRLYS
5   ALAGLULEUASNGLYLYSASPGLYPHEILE
6   PROLYSASNTYRILEGLUMETLYSPROHIS
7   PROTRPPHEPHEGLYLYSILEPROARGALA
8   LYSALAGLUGLUMETLEUSERLYSGLNARG
9   HISASPGLYALAPHELEUILEARGGLUSER
10   GLUSERALAPROGLYASPPHESERLEUSER
11   VALLYSPHEGLYASNASPVALGLNHISPHE
12   LYSVALLEUARGASPGLYALAGLYLYSTYR
13   PHELEUTRPVALVALLYSPHEASNSERLEU
14   ASNGLULEUVALASPTYRHISARGSERTHR
15   SERVALSERARGASNGLNGLNILEPHELEU
16   ARGASPILEGLUGLNVALPROGLNGLNPRO
17   THRTYRVALGLNALALEUPHEASPPHEASP
18   PROGLNGLUASPGLYGLULEUGLYPHEARG
19   ARGGLYASPPHEILEHISVALMETASPASN
20   SERASPPROASNTRPTRPLYSGLYALACYS
21   HISGLYGLNTHRGLYMETPHEPROARGASN
22   TYRVALTHRPROVALASNARGASNVAL

Samples:

sample_1: GRB2, [U-98% 13C; U-98% 15N;U-80% 2H]], 0.3 mM; PIPES, 12C, 14N, 1H, 40 mM; sodium chloride, 23Na, 35Cl, 150 mM; TCEP, 12C, 31P, 2 mM

sample_conditions_1: pH: 7.2; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.6, Bruker Biospin - NMR experiments recording and processing

CcpNMR v2.4, CCPN - data analysis

NMR spectrometers:

  • Bruker Avance 950 MHz
  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz

Related Database Links:

Uniprot P62993
AlphaFold Q63059

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks