BMRB Entry 50080

Name: Backbone 1H, 13C and 15N resonance assignment of USP7 TRAF-like domain free and in complex with DNA polymerase iota
Published: 2020-11-08

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50080

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C and 15N resonance assignment of USP7 TRAF-like domain free and in complex with DNA polymerase iota

Deposition date:

2019-10-24

Original release date:

2020-11-08

Authors:

Valles, Gabrielle; Bezsonova, Irina

Citation:

Citation: Ashton, Nicholas; Valles, Gabrielle; Jaiswal, Nancy; Bezsonova, Irina; Woodgate, Roger. "DNA Polymerase \u03b9 Interacts with Both the TRAF-like and UBL1-2 Domains of USP7." J. Mol. Biol. 433, 166733-166733 (2021).
PubMed: 33279577

Assembly members:

Assembly members:
entity_1, polymer, 145 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet-28b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GDTSWRSEATFQFTVERFSR LSESVLSPPCFVRNLPWKIM VMPRFYPDRPHQKSVGFFLQ CNAESDSTSWSCHAQAVLKI INYRDDEKSFSRRISHLFFH KENDWGFSNFMAWSEVTDPE KGFIDDDKVTFEVFVQADAP HGVAW

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1
- assigned_chemical_shifts_2
spectral_peak_list

Data type	Count
13C chemical shifts	335
15N chemical shifts	228
1H chemical shifts	228

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	USP7 TRAF-like domain	1

Entities:

Entity 1, USP7 TRAF-like domain 145 residues - Formula weight is not available

1

GLY

ASP

THR

SER

TRP

ARG

SER

GLU

ALA

THR

2

PHE

GLN

PHE

THR

VAL

GLU

ARG

PHE

SER

ARG

3

LEU

SER

GLU

SER

VAL

LEU

SER

PRO

CYS

4

PHE

VAL

ARG

ASN

LEU

PRO

TRP

LYS

ILE

MET

5

VAL

MET

PRO

ARG

PHE

TYR

PRO

ASP

ARG

PRO

6

HIS

GLN

LYS

SER

VAL

GLY

PHE

LEU

GLN

7

CYS

ASN

ALA

GLU

SER

ASP

SER

THR

SER

TRP

8

SER

CYS

HIS

ALA

GLN

ALA

VAL

LEU

LYS

ILE

9

ILE

ASN

TYR

ARG

ASP

GLU

LYS

SER

PHE

10

SER

ARG

ILE

SER

HIS

LEU

PHE

HIS

11

LYS

GLU

ASN

ASP

TRP

GLY

PHE

SER

ASN

PHE

12

MET

ALA

TRP

SER

GLU

VAL

THR

ASP

PRO

GLU

13

LYS

GLY

PHE

ILE

ASP

LYS

VAL

THR

14

PHE

GLU

VAL

PHE

VAL

GLN

ALA

ASP

ALA

PRO

15

HIS

GLY

VAL

ALA

TRP

Samples:

sample_1: entity_1, [U-100% 13C; U-100% 15N], 0.9 mM; sodium phosphate 20 mM; sodium chloride 250 mM; DTT 10 mM; D2O, [U-100% 2H], 10 % v/v

sample_2: entity_1, [U-100% 15N], 0.250 mM; sodium phosphate 20 mM; sodium chloride 250 mM; DTT 10 mM; D2O, [U-100% 2H], 10 % v/v; DNA polymerase iota peptide 573-584 1.25 mM

sample_conditions_1: ionic strength: 0.250 M; pH: 7.5; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_2	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRbox, UConn Health Center - data analysis

SPARKY, Goddard - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

Agilent INOVA 500 MHz
Agilent INOVA 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks