BMRB Entry 50079

Name: 1H, 15N, 13C assignment of BRCA2 190-284
Published: 2019-10-29

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR50079

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N, 13C assignment of BRCA2 190-284

Deposition date:

2019-10-23

Original release date:

2019-10-29

Authors:

Julien, Manon; Miron, Simona; Carreira, Aura; Theillet, Francois-Xavier; Zinn-Justin, Sophie

Citation:

Citation: Julien, Manon; Miron, Simona; Carreira, Aura; Theillet, Francois-Xavier; Zinn-Justin, Sophie. "1H, 13C and 15N backbone resonance assignment of the human BRCA2 N-terminal region" Biomol. NMR Assignments 14, 79-85 (2020).
PubMed: 31900740

Assembly members:

Assembly members:
entity_1, polymer, 96 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-6P-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GPDMSWSSSLATPPTLSSTV LIVRNEEASETVFPHDTTAN VKSYFSNHDESLKKNDRFIA SVTDSENTNQREAASHGFGK TSGNSFKVNSCKDHIG

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	269
15N chemical shifts	88
1H chemical shifts	86

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	BRCA2 190-284	1

Entities:

Entity 1, BRCA2 190-284 96 residues - Formula weight is not available

1

GLY

PRO

ASP

MET

SER

TRP

SER

LEU

2

ALA

THR

PRO

THR

LEU

SER

THR

VAL

3

LEU

ILE

VAL

ARG

ASN

GLU

ALA

SER

GLU

4

THR

VAL

PHE

PRO

HIS

ASP

THR

ALA

ASN

5

VAL

LYS

SER

TYR

PHE

SER

ASN

HIS

ASP

GLU

6

SER

LEU

LYS

ASN

ASP

ARG

PHE

ILE

ALA

7

SER

VAL

THR

ASP

SER

GLU

ASN

THR

ASN

GLN

8

ARG

GLU

ALA

SER

HIS

GLY

PHE

GLY

LYS

9

THR

SER

GLY

ASN

SER

PHE

LYS

VAL

ASN

SER

10

CYS

LYS

ASP

HIS

ILE

GLY

Samples:

sample_1: BRCA2 190-284, [U-95% 13C; U-95% 15N], 0.4 mM; HEPES 50 mM; EDTA 1 mM; DTT 2 mM; D2O 10%; DSS 50 uM

sample_conditions_1: ionic strength: 0 M; pH: 7.0; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR v2.4, Bruker Biospin, CCPN - chemical shift assignment, collection

NMR spectrometers:

Bruker Avance 600 MHz

UNP	P51587
AlphaFold	Q5TBJ7