BMRB Entry 50078

Name: 1H, 15N and 13C assignment of BRCA2 53-131
Published: 2019-10-29

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR50078

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N and 13C assignment of BRCA2 53-131

Deposition date:

2019-10-23

Original release date:

2019-10-29

Authors:

Julien, Manon; Miron, Simona; Carreira, Aura; Theillet, Francois-Xavier; Zinn-Justin, Sophie

Citation:

Citation: Julien, Manon; Miron, Simona; Carreira, Aura; Theillet, Francois-Xavier; Zinn-Justin, Sophie. "1H, 13C and 15N backbone resonance assignment of the human BRCA2 N-terminal region" Biomol. NMR Assignments 14, 79-85 (2020).
PubMed: 31900740

Assembly members:

Assembly members:
entity_1, polymer, 85 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-41b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GEESEHKNNNYEPNLFKTPQ RKPSYNQLASTPIIFKEQGL TLPLYQSPVKELDKFKLDLG RNVPNSRHKSLRTVKTKMDQ ADDVS

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	215
15N chemical shifts	68
1H chemical shifts	68

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	BRCA2 53-131	1

Entities:

Entity 1, BRCA2 53-131 85 residues - Formula weight is not available

1

GLY

GLU

SER

GLU

HIS

LYS

ASN

2

TYR

GLU

PRO

ASN

LEU

PHE

LYS

THR

PRO

GLN

3

ARG

LYS

PRO

SER

TYR

ASN

GLN

LEU

ALA

SER

4

THR

PRO

ILE

PHE

LYS

GLU

GLN

GLY

LEU

5

THR

LEU

PRO

LEU

TYR

GLN

SER

PRO

VAL

LYS

6

GLU

LEU

ASP

LYS

PHE

LYS

LEU

ASP

LEU

GLY

7

ARG

ASN

VAL

PRO

ASN

SER

ARG

HIS

LYS

SER

8

LEU

ARG

THR

VAL

LYS

THR

LYS

MET

ASP

GLN

9

ALA

ASP

VAL

SER

Samples:

sample_1: brca2 53-131, [U-95% 13C; U-95% 15N], 0.2 mM; HEPES 50 mM; EDTA 1 mM; DTT 2 mM; DSS 50 uM; D2O, [U-99% 2H], 10%

sample_conditions_1: ionic strength: 0 M; pH: 7.0; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCACB	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
2D HSQC	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR v2.4, Bruker Biospin, CCPN - chemical shift assignment, collection

NMR spectrometers:

Bruker Avance 600 MHz

UNP	P51587
AlphaFold	Q5TBJ7