BMRB Entry 50077

Name: 1H, 15N and 13C assignment of BRCA2 48-218(C4A)
Published: 2019-10-29

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR50077

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

1H, 15N and 13C assignment of BRCA2 48-218(C4A)

Deposition date:

2019-10-23

Original release date:

2019-10-29

Authors:

Julien, Manon; Miron, Simona; Carreira, Aura; Theillet, Francois-Xavier; Zinn-Justin, Sophie

Citation:

Citation: Julien, Manon; Miron, Simona; Carreira, Aura; Theillet, Francois-Xavier; Zinn-Justin, Sophie. "1H, 13C and 15N backbone resonance assignment of the human BRCA2 N-terminal region" Biomol. NMR Assignments 14, 79-85 (2020).
PubMed: 31900740

Assembly members:

Assembly members:
entity_1, polymer, 172 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM13

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GEESEHKNNNYEPNLFKTPQ RKPSYNQLASTPIIFKEQGL TLPLYQSPVKELDKFKLDLG RNVPNSRHKSLRTVKTKMDQ ADDVSAPLLNSALSESPVVL QATHVTPQRDKSVVAGSLFH TPKFVKGRQTPKHISESLGA EVDPDMSWSSSLATPPTLSS TVLIVRNEEASE

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	496
15N chemical shifts	152
1H chemical shifts	152

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	BRCA2 48-218(C4A)	1

Entities:

Entity 1, BRCA2 48-218(C4A) 172 residues - Formula weight is not available

1

GLY

GLU

SER

GLU

HIS

LYS

ASN

2

TYR

GLU

PRO

ASN

LEU

PHE

LYS

THR

PRO

GLN

3

ARG

LYS

PRO

SER

TYR

ASN

GLN

LEU

ALA

SER

4

THR

PRO

ILE

PHE

LYS

GLU

GLN

GLY

LEU

5

THR

LEU

PRO

LEU

TYR

GLN

SER

PRO

VAL

LYS

6

GLU

LEU

ASP

LYS

PHE

LYS

LEU

ASP

LEU

GLY

7

ARG

ASN

VAL

PRO

ASN

SER

ARG

HIS

LYS

SER

8

LEU

ARG

THR

VAL

LYS

THR

LYS

MET

ASP

GLN

9

ALA

ASP

VAL

SER

ALA

PRO

LEU

ASN

10

SER

ALA

LEU

SER

GLU

SER

PRO

VAL

LEU

11

GLN

ALA

THR

HIS

VAL

THR

PRO

GLN

ARG

ASP

12

LYS

SER

VAL

ALA

GLY

SER

LEU

PHE

HIS

13

THR

PRO

LYS

PHE

VAL

LYS

GLY

ARG

GLN

THR

14

PRO

LYS

HIS

ILE

SER

GLU

SER

LEU

GLY

ALA

15

GLU

VAL

ASP

PRO

ASP

MET

SER

TRP

SER

16

SER

LEU

ALA

THR

PRO

THR

LEU

SER

17

THR

VAL

LEU

ILE

VAL

ARG

ASN

GLU

ALA

18

SER

GLU

Samples:

sample_1: DSS 50 uM; D2O, [U-99% 2H], 5%; BRCA2 48-218(C4A), [U-95% 13C; U-95% 15N], 0.25 mM; HEPES 50 mM; EDTA 1 mM; DTT 2 mM

sample_conditions_1: ionic strength: 0 M; pH: 6.4; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR v2.4, Bruker Biospin, CCPN - chemical shift assignment, collection

NMR spectrometers:

Bruker Avance 600 MHz

UNP	P51587
AlphaFold	Q5TBJ7