BMRB Entry 50075

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50075

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Membrane-bound human PD-L1 cytoplasmic domain
Deposition date:
2019-10-17
Original release date:
2020-04-23
Authors:
Wen, Maorong; Wu, Bin; Cao, Yunlei; Ouyang, Bo
Citation:

Citation: Wen, Maorong; Wu, Bin; Cao, Yunlei; Ouyang, Bo. "Membrane-bound human PD-L1 cytoplasmic domain"  .

Assembly members:

Assembly members:
entity_1, polymer, 33 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28-a-SUMO

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GPRLRKGRMMDVKKCGIQDT NSKKQSDTHLEET

Data sets:
Data typeCount
13C chemical shifts134
15N chemical shifts37
1H chemical shifts223

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 33 residues - Formula weight is not available

1   GLYPROARGLEUARGLYSGLYARGMETMET
2   ASPVALLYSLYSCYSGLYILEGLNASPTHR
3   ASNSERLYSLYSGLNSERASPTHRHISLEU
4   GLUGLUTHR

Samples:

sample_1: hPD-L1-CD, [U-13C; U-15N; U-2H], 0.5 mM; DMPG 60 mM; DHPC 85 mM; MES 25 mM; D2O, [U-2H], 10%; H2O 90%

sample_2: hPD-L1-CD, [U-99% 13C; U-99% 15N], 0.5 mM; DMPG, [U-2H], 60 mM; DHPC, [U-2H], 85 mM; MES 25 mM; D2O, [U-2H], 10%; H2O 90%

sample_3: hPD-L1-CD, [U-15N; U-2H], 0.5 mM; DMPG 60 mM; DHPC, [U-2H], 85 mM; MES 25 mM; D2O, [U-2H], 10%; H2O 90%

sample_conditions_1: ionic strength: 25 mM; pH: 6.5; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1
3D 1H-15N NOESY (NOE to lipid)sample_3isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D CCH-TOCSYsample_2isotropicsample_conditions_1

Software:

TOPSPIN v3.5.5, Bruker Biospin - collection

NMRPipe v8.9, Delaglio, Zhengrong and Bax - processing

X-PLOR v2.44, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution

CYANA, Guntert, Mumenthaler and Wuthrich - data analysis

SPARKY, Goddard - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Agilent DD2 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks