BMRB Entry 50052

Name: 1H, 13C, and 15N Chemical Shift Assignments for hairpin RNA Rest04
Published: 2021-02-02

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50052

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, and 15N Chemical Shift Assignments for hairpin RNA Rest04

Deposition date:

2019-10-09

Original release date:

2021-02-02

Authors:

Wang, Yanjiao; Han, Ge; Jiang, Xiuying; Yuwen, Tairan; Al-Hashimi, Hashim; Xue, Yi

Citation:

Citation: Wang, Yanjiao; Han, Ge; Jiang, Xiuying; Yuwen, Tairan; Xue, Yi. "Chemical shift prediction of RNA imino groups: application toward characterizing RNA excited states" Nat. Commun. 12, 1595-1595 (2021).
PubMed: 33707433

Assembly members:

Assembly members:
hairpin RNA Rest04, polymer, 30 residues, Formula weight is not available

Natural source:

Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available

Experimental source:

Experimental source: Production method: in vitro transcription Host organism: in vitro transcription

Entity Sequences (FASTA):

Entity Sequences (FASTA):
hairpin RNA Rest04: GGUCUUGAAAUACUUCGGUA UUUUGGGGCC

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
15N chemical shifts	18
1H chemical shifts	18

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Rest04	1

Entities:

Entity 1, Rest04 30 residues - Formula weight is not available

1	G	G	U	C	U	U	G	A	A	A
2	U	A	C	U	U	C	G	G	U	A
3	U	U	U	U	G	G	G	G	C	C

Samples:

sample_1: RNA(30-mer) 2.29 mM; sodium phosphate 10 mM; EDTA 0.01 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 10 mM; pH: 6.4; pressure: 1 atm; temperature: 283.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HMQC	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

SPARKY, Goddard - chemical shift assignment, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Bruker AVANCE III HD 600MHz MHz
Bruker AVANCE III HD 800MHZ MHz