BMRB Entry 5005

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5005

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone Sequential Resonance Assignments of Yeast iso-2 Cytochrome c, Reduced and Oxidized forms

Deposition date:

2001-05-01

Original release date:

2002-06-26

Authors:

Benavides-Garcia, Maria; Rivera, Edna; Ramos, William; Hinck, Andrew; Nall, Barry

Citation:

Citation: Benavides-Garcia, Maria; Rivera, Edna; Ramos, William; Hinck, Andrew; Nall, Barry. "Letter to the Editor: Backbone Sequential Resonance Assignments of Yeast iso-2 Cytochrome c, Reduced and Oxidized forms" J. Biomol. NMR 22, 93-94 (2002).
PubMed: 11885986

Assembly members:

Assembly members:
yeast iso-2 cytochrome c, oxidized form, polymer, 112 residues, 12958 Da.
HEC, non-polymer, 618.503 Da.

Natural source:

Natural source: Common Name: Baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pERICYC7

Entity Sequences (FASTA):

Entity Sequences (FASTA):
yeast iso-2 cytochrome c, oxidized form: AKESTGFKPGSAKKGATLFK TRCQQCHTIEEGGPNKVGPN LHGIFGRHSGQVKGYSYTDA NINKNVKWDEDSMSEYLTNP AKYIPGTKMAFAGLKKEKDR NDLITYMTKAAK

Data sets:

assigned_chemical_shifts
- csr1

Data type	Count
1H chemical shifts	360
13C chemical shifts	418
15N chemical shifts	104

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	yeast iso-2 cyt c	1
2	oxidized heme	2

Entities:

Entity 1, yeast iso-2 cyt c 112 residues - 12958 Da.

1

ALA

LYS

GLU

SER

THR

GLY

PHE

LYS

PRO

GLY

2

SER

ALA

LYS

GLY

ALA

THR

LEU

PHE

LYS

3

THR

ARG

CYS

GLN

CYS

HIS

THR

ILE

GLU

4

GLU

GLY

PRO

ASN

LYS

VAL

GLY

PRO

ASN

5

LEU

HIS

GLY

ILE

PHE

GLY

ARG

HIS

SER

GLY

6

GLN

VAL

LYS

GLY

TYR

SER

TYR

THR

ASP

ALA

7

ASN

ILE

ASN

LYS

ASN

VAL

LYS

TRP

ASP

GLU

8

ASP

SER

MET

SER

GLU

TYR

LEU

THR

ASN

PRO

9

ALA

LYS

TYR

ILE

PRO

GLY

THR

LYS

MET

ALA

10

PHE

ALA

GLY

LEU

LYS

GLU

LYS

ASP

ARG

11

ASN

ASP

LEU

ILE

THR

TYR

MET

THR

LYS

ALA

12

ALA

LYS

Entity 2, oxidized heme - C34 H34 Fe N4 O4 - 618.503 Da.

1

HEC

Samples:

sample1: yeast iso-2 cytochrome c, oxidized form, [U-13C; U-15N], 2.0 mM

condition1: pH: 6.0; temperature: 293 K; ionic strength: 0.13 M

Experiments:

Name	Sample	Sample state	Sample conditions
HSQC COSY	sample1	not available	condition1
HNCACB	sample1	not available	condition1
C(CO)NH	sample1	not available	condition1
HNCO	sample1	not available	condition1
HN(CA)CO	sample1	not available	condition1
HBHA(CO)NH	sample1	not available	condition1

Software:

NMRPipe v1.8 - processing

NMR spectrometers:

Bruker AMX 500 MHz

REF	NP_010875
SWISS-PROT	P00045
GenBank	AAD13974 AAT93051 AAB59339 AAB65003 AAA34940
EMBL	CAA24606
PDB	1YTC 3CXH 1YEA
BMRB	5003