BMRB Entry 50035

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for dL3D1
Deposition date:
2019-10-06
Original release date:
2021-06-13
Authors:
Zhang, Shengnan
Citation:

Citation: Zhang, Shengnan; Liu, Yuqing; Jia, Chunyu; Lim, Yeh-Jun; Feng, Guoqin; Xu, Enquan; Kimura, Yasuyoshi; Long, Houfang; Tao, Youqi; Zhao, Chunyu; Wang, Chuchu; Liu, Zhenying; Hu, Jin-Jian; Ma, Mengrong; Jiang, Lin; Li, Dan; Wang, Renxiao; Dawson, Valina; Dawson, Ted; Li, Yan-Mei; Mao, Xiaobo; Liu, Cong. "Backbone 1H, 13C, and 15N Chemical Shift Assignments for dL3D1"  PNAS ., .-..

Assembly members:

Assembly members:
dL3D1, polymer, 125 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a(+)

Data sets:
Data typeCount
13C chemical shifts215
15N chemical shifts100
1H chemical shifts100

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1dL3D11

Entities:

Entity 1, dL3D1 125 residues - Formula weight is not available

1   GLNGLYSERGLYPROGLYLYSGLULEUPRO
2   VALVALTRPALAGLNGLUGLYALAPROVAL
3   HISLEUPROCYSSERLEULYSSERPROASN
4   LEUASPPROASNPHELEUARGARGGLYGLY
5   VALILETRPGLNHISGLNPROASPSERGLY
6   GLNPROTHRGLYARGTYRTHRVALLEUSER
7   VALALAPROGLYGLYLEUARGSERGLYARG
8   GLNPROLEUHISPROHISVALGLNLEUGLU
9   GLUARGGLYLEUGLNARGGLYASPPHESER
10   LEUTRPLEUARGPROALALEUARGTHRASP
11   ALAGLYGLUTYRHISALATHRVALARGLEU
12   PROASNARGALALEUSERCYSSERLEUARG
13   LEUARGVALGLYGLN

Samples:

sample_1: sodium phosphate 25 mM; sodium chloride 50 mM; D2O, [U-2H], 10%; entity_1, [U-99% 13C; U-99% 15N], 150 mM

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks