BMRB Entry 50034

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for APLP1 E1 domain
Published: 2021-06-13

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50034

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for APLP1 E1 domain

Deposition date:

2019-10-05

Original release date:

2021-06-13

Authors:

Zhang, Shengnan

Citation:

Citation: Zhang, Shengnan; Liu, Yuqing; Jia, Chunyu; Lim, Yeh-Jun; Feng, Guoqin; Xu, Enquan; Kimura, Yasuyoshi; Long, Houfang; Tao, Youqi; Zhao, Chunyu; Wang, Chuchu; Liu, Zhenying; Hu, Jin-Jian; Ma, Mengrong; Jiang, Lin; Li, Dan; Wang, Renxiao; Dawson, Valina; Dawson, Ted; Li, Yan-Mei; Mao, Xiaobo; Liu, Cong. "Backbone 1H, 13C, and 15N Chemical Shift Assignments for APLP1 E1 domain" PNAS ., .-..

Assembly members:

Assembly members:
A1E1, polymer, 148 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28-a(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
A1E1: QGMGPASPAARGLSRRPGQP PLPLLLPLLLLLLRAQPAIG SLAGGSPGAAEAPGSAQVAG LCGRLTLHRDLRTGRWEPDP QRSRRCLRDPQRVLEYCRQM YPELQIARVEQATQAIPMER WCGGSRSGSCAHPHHQVVPF RCLPGEFV

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	155
15N chemical shifts	70
1H chemical shifts	70

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	APLP1 E1 domain	1

Entities:

Entity 1, APLP1 E1 domain 148 residues - Formula weight is not available

1

GLN

GLY

MET

GLY

PRO

ALA

SER

PRO

ALA

2

ARG

GLY

LEU

SER

ARG

PRO

GLY

GLN

PRO

3

PRO

LEU

PRO

LEU

PRO

LEU

4

LEU

ARG

ALA

GLN

PRO

ALA

ILE

GLY

5

SER

LEU

ALA

GLY

SER

PRO

GLY

ALA

6

GLU

ALA

PRO

GLY

SER

ALA

GLN

VAL

ALA

GLY

7

LEU

CYS

GLY

ARG

LEU

THR

LEU

HIS

ARG

ASP

8

LEU

ARG

THR

GLY

ARG

TRP

GLU

PRO

ASP

PRO

9

GLN

ARG

SER

ARG

CYS

LEU

ARG

ASP

PRO

10

GLN

ARG

VAL

LEU

GLU

TYR

CYS

ARG

GLN

MET

11

TYR

PRO

GLU

LEU

GLN

ILE

ALA

ARG

VAL

GLU

12

GLN

ALA

THR

GLN

ALA

ILE

PRO

MET

GLU

ARG

13

TRP

CYS

GLY

SER

ARG

SER

GLY

SER

CYS

14

ALA

HIS

PRO

HIS

GLN

VAL

PRO

PHE

15

ARG

CYS

LEU

PRO

GLY

GLU

PHE

VAL

Samples:

sample_1: sodium phosphate 25 mM; sodium chloride 50 mM; D2O 10 % v/v

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Agilent DD2 600 MHz
Agilent DD2 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks