BMRB Entry 50024

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50024

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Title:
Smurf1 HECT
Deposition date:
2019-09-23
Original release date:
2020-09-21
Authors:
Wiesner, Silke
Citation:

Citation: Ruetalo, Natalia; Anders, Samira; Stollmaier, Carsten; Jaeckl, Magnus; Schuetz-Stoffregen, Mira; Stefan, Nadine; Wolf, Christine; Wiesner, Silke. "The WW1 Domain Enhances Autoinhibition in Smurf Ubiquitin Ligases"  J. Mol. Biol. 431, 4834-4847 (2019).
PubMed: 31628949

Assembly members:

Assembly members:
sf1hect, polymer, 731 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pProExHTb

Entity Sequences (FASTA):

Entity Sequences (FASTA):
sf1hect: MSNPGTRRNGSSIKIRLTVL CAKNLAKKDFFRLPDPFAKI VVDGSGQCHSTDTVKNTLDP KWNQHYDLYVGKTDSITISV WNHKKIHKKQGAGFLGCVRL LSNAISRLKDTGYQRLDLCK LNPSDTDAVRGQIVVSLQTR DRIGTGGSVVDCRGLLENEG TVYEDSGPGRPLSCFMEEPA PYTDSTGAAAGGGNCRFVES PSQDQRLQAQRLRNPDVRGS LQTPQNRPHGHQSPELPEGY EQRTTVQGQVYFLHTQTGVS TWHDPRIPRDLNSVNCDELG PLPPGWEVRSTVSGRIYFVD HNNRTTQFTDPRLHHIMNHQ CQLKEPSQPLPLPSEGSLED EELPAQRYERDLVQKLKVLR HELSLQQPQAGHCRIEVSRE EIFEESYRQIMKMRPKDLKK RLMVKFRGEEGLDYGGVARE WLYLLCHEMLNPYYGLFQYS TDNIYMLQINPDSSINPDHL SYFHFVGRIMGLAVFHGHYI NGGFTVPFYKQLLGKPIQLS DLESVDPELHKSLVWILEND ITPVLDHTFCVEHNAFGRIL QHELKPNGRNVPVTEENKKE YVRLYVNWRFMRGIEAQFLA LQKGFNELIPQHLLKPFDQK ELELIIGGLDKIDLNDWKSN TRLKHCVADSNIVRWFWQAV ETFDEERRARLLQFVTGSTR VPLQGFKALQGSTGAAGPRL FTIHLIDANTDNLPKAHTCF NRIDIPPYESYEKLYEKLLT AVEETCGFAVE

Data sets:
Data typeCount
13C chemical shifts28
1H chemical shifts84

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1sf1hect1

Entities:

Entity 1, sf1hect 731 residues - Formula weight is not available

1   METSERASNPROGLYTHRARGARGASNGLY
2   SERSERILELYSILEARGLEUTHRVALLEU
3   CYSALALYSASNLEUALALYSLYSASPPHE
4   PHEARGLEUPROASPPROPHEALALYSILE
5   VALVALASPGLYSERGLYGLNCYSHISSER
6   THRASPTHRVALLYSASNTHRLEUASPPRO
7   LYSTRPASNGLNHISTYRASPLEUTYRVAL
8   GLYLYSTHRASPSERILETHRILESERVAL
9   TRPASNHISLYSLYSILEHISLYSLYSGLN
10   GLYALAGLYPHELEUGLYCYSVALARGLEU
11   LEUSERASNALAILESERARGLEULYSASP
12   THRGLYTYRGLNARGLEUASPLEUCYSLYS
13   LEUASNPROSERASPTHRASPALAVALARG
14   GLYGLNILEVALVALSERLEUGLNTHRARG
15   ASPARGILEGLYTHRGLYGLYSERVALVAL
16   ASPCYSARGGLYLEULEUGLUASNGLUGLY
17   THRVALTYRGLUASPSERGLYPROGLYARG
18   PROLEUSERCYSPHEMETGLUGLUPROALA
19   PROTYRTHRASPSERTHRGLYALAALAALA
20   GLYGLYGLYASNCYSARGPHEVALGLUSER
21   PROSERGLNASPGLNARGLEUGLNALAGLN
22   ARGLEUARGASNPROASPVALARGGLYSER
23   LEUGLNTHRPROGLNASNARGPROHISGLY
24   HISGLNSERPROGLULEUPROGLUGLYTYR
25   GLUGLNARGTHRTHRVALGLNGLYGLNVAL
26   TYRPHELEUHISTHRGLNTHRGLYVALSER
27   THRTRPHISASPPROARGILEPROARGASP
28   LEUASNSERVALASNCYSASPGLULEUGLY
29   PROLEUPROPROGLYTRPGLUVALARGSER
30   THRVALSERGLYARGILETYRPHEVALASP
31   HISASNASNARGTHRTHRGLNPHETHRASP
32   PROARGLEUHISHISILEMETASNHISGLN
33   CYSGLNLEULYSGLUPROSERGLNPROLEU
34   PROLEUPROSERGLUGLYSERLEUGLUASP
35   GLUGLULEUPROALAGLNARGTYRGLUARG
36   ASPLEUVALGLNLYSLEULYSVALLEUARG
37   HISGLULEUSERLEUGLNGLNPROGLNALA
38   GLYHISCYSARGILEGLUVALSERARGGLU
39   GLUILEPHEGLUGLUSERTYRARGGLNILE
40   METLYSMETARGPROLYSASPLEULYSLYS
41   ARGLEUMETVALLYSPHEARGGLYGLUGLU
42   GLYLEUASPTYRGLYGLYVALALAARGGLU
43   TRPLEUTYRLEULEUCYSHISGLUMETLEU
44   ASNPROTYRTYRGLYLEUPHEGLNTYRSER
45   THRASPASNILETYRMETLEUGLNILEASN
46   PROASPSERSERILEASNPROASPHISLEU
47   SERTYRPHEHISPHEVALGLYARGILEMET
48   GLYLEUALAVALPHEHISGLYHISTYRILE
49   ASNGLYGLYPHETHRVALPROPHETYRLYS
50   GLNLEULEUGLYLYSPROILEGLNLEUSER
51   ASPLEUGLUSERVALASPPROGLULEUHIS
52   LYSSERLEUVALTRPILELEUGLUASNASP
53   ILETHRPROVALLEUASPHISTHRPHECYS
54   VALGLUHISASNALAPHEGLYARGILELEU
55   GLNHISGLULEULYSPROASNGLYARGASN
56   VALPROVALTHRGLUGLUASNLYSLYSGLU
57   TYRVALARGLEUTYRVALASNTRPARGPHE
58   METARGGLYILEGLUALAGLNPHELEUALA
59   LEUGLNLYSGLYPHEASNGLULEUILEPRO
60   GLNHISLEULEULYSPROPHEASPGLNLYS
61   GLULEUGLULEUILEILEGLYGLYLEUASP
62   LYSILEASPLEUASNASPTRPLYSSERASN
63   THRARGLEULYSHISCYSVALALAASPSER
64   ASNILEVALARGTRPPHETRPGLNALAVAL
65   GLUTHRPHEASPGLUGLUARGARGALAARG
66   LEULEUGLNPHEVALTHRGLYSERTHRARG
67   VALPROLEUGLNGLYPHELYSALALEUGLN
68   GLYSERTHRGLYALAALAGLYPROARGLEU
69   PHETHRILEHISLEUILEASPALAASNTHR
70   ASPASNLEUPROLYSALAHISTHRCYSPHE
71   ASNARGILEASPILEPROPROTYRGLUSER
72   TYRGLULYSLEUTYRGLULYSLEULEUTHR
73   ALAVALGLUGLUTHRCYSGLYPHEALAVAL
74   GLU

Samples:

sample_1: Smurf1 HECT domain, 1H,13C-Ile,Met methyl, 0.04 mM; sodium phosphate 20 mM; sodium chloride 150 mM; DTT 1 mM

sample_conditions_1: ionic strength: 0.5 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HMQCsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz