BMRB Entry 50021

Name: Backbone NMR assignments of the Smurf2 WW1 domain
Published: 2020-09-21

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50021

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone NMR assignments of the Smurf2 WW1 domain

Deposition date:

2019-09-23

Original release date:

2020-09-21

Authors:

Wiesner, Silke

Citation:

Citation: Ruetalo, Natalia; Anders, Samira; Stollmaier, Carsten; Jaeckl, Magnus; Schuetz-Stoffregen, Mira; Stefan, Nadine; Wolf, Christine; Wiesner, Silke. "The WW1 Domain Enhances Autoinhibition in Smurf Ubiquitin Ligases" J. Mol. Biol. 431, 4834-4847 (2019).
PubMed: 31628949

Assembly members:

Assembly members:
Smurf2 WW1 domain, polymer, 41 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM-41

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Smurf2 WW1 domain: GAMGNDLPDGWEERRTASGR IQYLNHITRTTQWERPTRPA S

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	66
15N chemical shifts	29
1H chemical shifts	29

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	WW1	1

Entities:

Entity 1, WW1 41 residues - Formula weight is not available

1

GLY

ALA

MET

GLY

ASN

ASP

LEU

PRO

ASP

GLY

2

TRP

GLU

ARG

THR

ALA

SER

GLY

ARG

3

ILE

GLN

TYR

LEU

ASN

HIS

ILE

THR

ARG

THR

4

THR

GLN

TRP

GLU

ARG

PRO

THR

ARG

PRO

ALA

5

SER

Samples:

sample_1: sodium phosphate 20 mM; sodium chloride 150 mM; DTT 1 mM; WW1, [U-100% 13C], 1 mM

sample_conditions_1: ionic strength: 0.5 M; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

XEASY, Bartels et al. - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks