BMRB Entry 50020

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50020

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Title:
Structural and biophysical characterization of the type VII collagen vWFA2 subdomain leads to identification of two binding sites and helps understanding the consequences of autoimmunity against type VII collagen
Deposition date:
2019-09-23
Original release date:
2020-02-13
Authors:
Gebauer, Jan; Flachsenberg, Florian; Windler, Cordula; Richer, Barbara; Baumann, Ulrich; Seeger, Karsten
Citation:

Citation: Gebauer, Jan; Flachsenberg, Florian; Windler, Cordula; Richer, Barbara; Baumann, Ulrich; Seeger, Karsten. "Structural and biophysical characterization of the type VII collagen vWFA2 subdomain leads to identification of two binding sites"  FEBS Open Bio. 10, 580-592 (2020).
PubMed: 32031736

Assembly members:

Assembly members:
entity_1, polymer, 196 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pTWIN1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GRAMGACSHGPVDVVFLLHA TRDNAHNAEAVRRVLERLVS ALGPLGPQAAQVGLLTYSHR PSPLFPLNSSHDLGIILRKI RDIPYVDPSGNNLGTAVTTA HRYLLASNAPGRRQQVPGVM VLLVDEPLRGDILSPIREAQ TSGLKVMALSLVGADPEQLR RLAPGTDPIQNFFAVDNGPG LDRAVSDLAVALCQAA

Data sets:
Data typeCount
T1 relaxation values140
T2 relaxation values140
heteronuclear NOE values141
residual dipolar couplings288

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1mvWFA21

Entities:

Entity 1, mvWFA2 196 residues - Formula weight is not available

1   GLYARGALAMETGLYALACYSSERHISGLY
2   PROVALASPVALVALPHELEULEUHISALA
3   THRARGASPASNALAHISASNALAGLUALA
4   VALARGARGVALLEUGLUARGLEUVALSER
5   ALALEUGLYPROLEUGLYPROGLNALAALA
6   GLNVALGLYLEULEUTHRTYRSERHISARG
7   PROSERPROLEUPHEPROLEUASNSERSER
8   HISASPLEUGLYILEILELEUARGLYSILE
9   ARGASPILEPROTYRVALASPPROSERGLY
10   ASNASNLEUGLYTHRALAVALTHRTHRALA
11   HISARGTYRLEULEUALASERASNALAPRO
12   GLYARGARGGLNGLNVALPROGLYVALMET
13   VALLEULEUVALASPGLUPROLEUARGGLY
14   ASPILELEUSERPROILEARGGLUALAGLN
15   THRSERGLYLEULYSVALMETALALEUSER
16   LEUVALGLYALAASPPROGLUGLNLEUARG
17   ARGLEUALAPROGLYTHRASPPROILEGLN
18   ASNPHEPHEALAVALASPASNGLYPROGLY
19   LEUASPARGALAVALSERASPLEUALAVAL
20   ALALEUCYSGLNALAALA

Samples:

sample_1: mvWFA2, [U-99% 15N], 1 mM; potassium phosphate 10 mM; H2O 90 % v/v; D2O 10 % v/v

sample_2: mvWFA2, [U-100% 15N], 0.6 mM; sodium phosphate 10 mM; D2O, [U-2H], 10 % v/v; PEG 4.2 % w/v; 1-Hexanol 1.5 % v/v

sample_3: mvWFA2, [U-100% 15N], 0.6 mM; sodium phosphate 10 mM; D2O, [U-2H], 10 % v/v; PEG 4.2 % w/v; 1-Hexanol 1.9 % v/v; CTAB 0.1 % w/v

sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
15N-(1H) NOEsample_1isotropicsample_conditions_1
hsqct1etf3gpsi3dsample_1isotropicsample_conditions_1
hsqct2etf3gpsi3dsample_1isotropicsample_conditions_1
hsqcf3gpiaphsiwgsample_2isotropicsample_conditions_1
hsqcf3gpiaphsiwgsample_3isotropicsample_conditions_1
hsqcf3gpiaphsiwgsample_1isotropicsample_conditions_1

Software:

CcpNMR, CCPN - data analysis

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

  • Bruker DRX 500 MHz