BMRB Entry 50017

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for the hnRNPA1 LCD (deltaHexa construct)
Published: 2020-05-20

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50017

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for the hnRNPA1 LCD (deltaHexa construct)

Deposition date:

2019-09-13

Original release date:

2020-05-20

Authors:

Peran, Ivan

Citation:

Citation: Martin, Erik; Holehouse, Alex; Peran, Ivan; Farag, Mina; Incicco, J. Jeremias; Bremer, Anne; Grace, Christy; Soranno, Andrea; Pappu, Rohit; Mittag, Tanja. "Valence and Patterning of Aromatic Residues Determine the Phase Behavior of Prion-Like Domains" Science 367, 694-699 (2020).
PubMed: 32029630

Assembly members:

Assembly members:
A1 LCD (deltahexa), polymer, 131 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pDEST17

Entity Sequences (FASTA):

Entity Sequences (FASTA):
A1 LCD (deltahexa): GSMASASSSQRGRSGSGNFG GGRGGGFGGNDNFGRGGNFS GRGGFGGSRGGGGYGGSGDG YNGFGNDGSNFGGGGNYNNQ SSNFGPMKGGNFGGRSSGPY GGGGQYFAKPRNQGGYGGSS SSSSYGSGRRF

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	325
15N chemical shifts	125
1H chemical shifts	125

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Low complexity region (LCD)	1

Entities:

Entity 1, Low complexity region (LCD) 131 residues - Formula weight is not available

1

GLY

SER

MET

ALA

SER

ALA

SER

GLN

2

ARG

GLY

ARG

SER

GLY

SER

GLY

ASN

PHE

GLY

3

GLY

ARG

GLY

PHE

GLY

ASN

4

ASP

ASN

PHE

GLY

ARG

GLY

ASN

PHE

SER

5

GLY

ARG

GLY

PHE

GLY

SER

ARG

GLY

6

GLY

TYR

GLY

SER

GLY

ASP

GLY

7

TYR

ASN

GLY

PHE

GLY

ASN

ASP

GLY

SER

ASN

8

PHE

GLY

ASN

TYR

ASN

GLN

9

SER

ASN

PHE

GLY

PRO

MET

LYS

GLY

10

ASN

PHE

GLY

ARG

SER

GLY

PRO

TYR

11

GLY

GLN

TYR

PHE

ALA

LYS

PRO

12

ARG

ASN

GLN

GLY

TYR

GLY

SER

13

SER

TYR

GLY

SER

GLY

ARG

14

PHE

Samples:

sample_1: LCD 836 uM; D2O 10%; DSS 200 uM; H2O 90%; MES 50 mM; DTT 1 mM

sample_conditions_1: pH: 5.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D NH(CA)NNH	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Goddard, Keller and Wuthrich - collection

NMR spectrometers:

Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks