BMRB Entry 50011
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR50011
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Christou, Nina-Eleni; Ayala, Isabel; Giandoreggio-Barranco, Karine; Byrdin, Martin; Adam, Virgile; Bourgeois, Dominique; Brutscher, Bernhard. "NMR Reveals Light-Induced Changes in the Dynamics of a Photoswitchable Fluorescent Protein" Biophys. J. 117, 2087-2100 (2019).
PubMed: 31733726
Assembly members:
entity_1, polymer, 249 residues, Formula weight is not available
Natural source: Common Name: Aequorea victoria Taxonomy ID: 6100 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Aequorea victoria
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15-b_rsFolder
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 671 |
| 15N chemical shifts | 201 |
| 1H chemical shifts | 471 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | rsFolder | 1 |
Entities:
Entity 1, rsFolder 249 residues - Formula weight is not available
| 1 | GLY | SER | HIS | MET | ALA | THR | MET | VAL | SER | LYS | ||||
| 2 | GLY | GLU | GLU | LEU | PHE | THR | GLY | VAL | VAL | PRO | ||||
| 3 | ILE | LEU | VAL | GLU | LEU | ASP | GLY | ASP | VAL | ASN | ||||
| 4 | GLY | HIS | LYS | PHE | SER | VAL | ARG | GLY | GLU | GLY | ||||
| 5 | GLU | GLY | ASP | ALA | THR | ASN | GLY | LYS | LEU | THR | ||||
| 6 | LEU | LYS | PHE | ILE | CYS | THR | THR | GLY | LYS | LEU | ||||
| 7 | PRO | VAL | PRO | TRP | PRO | THR | LEU | VAL | THR | THR | ||||
| 8 | LEU | ALA | TYR | GLY | VAL | LEU | CYS | PHE | SER | ARG | ||||
| 9 | TYR | PRO | ASP | HIS | MET | LYS | ARG | HIS | ASP | PHE | ||||
| 10 | PHE | LYS | SER | ALA | MET | PRO | GLU | GLY | TYR | VAL | ||||
| 11 | GLN | GLU | ARG | THR | ILE | SER | PHE | LYS | ASP | ASP | ||||
| 12 | GLY | THR | TYR | LYS | THR | ARG | ALA | GLU | VAL | LYS | ||||
| 13 | PHE | GLU | GLY | ASP | THR | LEU | VAL | ASN | ARG | ILE | ||||
| 14 | GLU | LEU | LYS | GLY | ILE | ASP | PHE | LYS | GLU | ASP | ||||
| 15 | GLY | ASN | ILE | LEU | GLY | HIS | LYS | LEU | GLU | TYR | ||||
| 16 | ASN | PHE | ASN | SER | HIS | ASN | VAL | TYR | ILE | THR | ||||
| 17 | ALA | ASP | LYS | GLN | LYS | ASN | GLY | ILE | LYS | SER | ||||
| 18 | ASN | PHE | LYS | ILE | ARG | HIS | ASN | VAL | GLU | ASP | ||||
| 19 | GLY | SER | VAL | GLN | LEU | ALA | ASP | HIS | TYR | GLN | ||||
| 20 | GLN | ASN | THR | PRO | ILE | GLY | ASP | GLY | PRO | VAL | ||||
| 21 | LEU | LEU | PRO | ASP | ASN | HIS | TYR | LEU | SER | THR | ||||
| 22 | GLN | SER | LYS | LEU | SER | LYS | ASP | PRO | ASN | GLU | ||||
| 23 | LYS | ARG | ASP | HIS | MET | VAL | LEU | LEU | GLU | PHE | ||||
| 24 | VAL | THR | ALA | ALA | GLY | ILE | THR | HIS | GLY | MET | ||||
| 25 | ASP | GLU | LEU | TYR | LYS | GLY | SER | GLY | CYS |
Samples:
sample_1: entity_1, [U-100% 13C; U-100% 15N], 200 ± 20 uM; HEPES 50 mM; NaCl 100 mM
sample_conditions_1: pH: 7.50; pressure: 1 atm; temperature: 313 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CACB | sample_1 | isotropic | sample_conditions_1 |
| 3D Laser-Driven EXSY | sample_1 | isotropic | sample_conditions_1 |
| 2D AminoAcid_Edited 1H-13C SOFAST HMQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C SOFAST HMQC | sample_1 | isotropic | sample_conditions_1 |
Software:
software_1 v2.4.2, CCPN - chemical shift assignment
NMR spectrometers:
- Bruker Avance 700 MHz
- Bruker Avance 850 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks