BMRB Entry 50002

Name: Pof8 RNA recognition motif 2
Published: 2021-08-13

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50002

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Pof8 RNA recognition motif 2

Deposition date:

2019-08-18

Original release date:

2021-08-13

Authors:

Eichhorn, Catherine; Feigon, Juli

Citation:

Citation: Basu, Ritwika; Eichhorn, Catherine; Cheng, Ryan; Peterson, Robert; Feigon, Juli. "Structure of S. pombe telomerase protein Pof8 C-terminal domain is an xRRM conserved among LARP7 proteins" RNA Biol. 18, 1181-1192 (2021).
PubMed: 33131423

Assembly members:

Assembly members:
entity_1, polymer, 121 residues, Formula weight is not available

Natural source:

Natural source: Common Name: fission yeast Taxonomy ID: 4896 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Schizosaccharomyces pombe

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: KDEDNLDFTKNLLTRIKNLH PLTNKSTIHSLLSYVFSRQT QNIACEPMYIDYRKDETEAI IRWKTPLHAETCINAFRTQE RKQNSHDDIRAHRKKGSSRP FLIAELITGEEEKNYWRMLK K

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	276
15N chemical shifts	106
1H chemical shifts	131

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Pof8 C-terminal xRRM	1

Entities:

Entity 1, Pof8 C-terminal xRRM 121 residues - Formula weight is not available

1

LYS

ASP

GLU

ASP

ASN

LEU

ASP

PHE

THR

LYS

2

ASN

LEU

THR

ARG

ILE

LYS

ASN

LEU

HIS

3

PRO

LEU

THR

ASN

LYS

SER

THR

ILE

HIS

SER

4

LEU

SER

TYR

VAL

PHE

SER

ARG

GLN

THR

5

GLN

ASN

ILE

ALA

CYS

GLU

PRO

MET

TYR

ILE

6

ASP

TYR

ARG

LYS

ASP

GLU

THR

GLU

ALA

ILE

7

ILE

ARG

TRP

LYS

THR

PRO

LEU

HIS

ALA

GLU

8

THR

CYS

ILE

ASN

ALA

PHE

ARG

THR

GLN

GLU

9

ARG

LYS

GLN

ASN

SER

HIS

ASP

ILE

ARG

10

ALA

HIS

ARG

LYS

GLY

SER

ARG

PRO

11

PHE

LEU

ILE

ALA

GLU

LEU

ILE

THR

GLY

GLU

12

GLU

LYS

ASN

TYR

TRP

ARG

MET

LEU

LYS

13

LYS

Samples:

sample_1: Pof8 C-terminal xRRM, [U-13C; U-15N], 0.8 mM; sodium phosphate 20 mM; potassium chloride 50 mM; TCEP 1 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.1; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
15N-(1H) NOE	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment, data analysis

TOPSPIN, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

PINE, Bahrami, Markley, Assadi, and Eghbalnia - chemical shift assignment

NMR spectrometers:

Bruker DRX 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks