BMRB Entry 4999
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4999
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Citation: Marion, Dominique; Tarbouriech, Nicolas; Ruigrok, Rob; Burmeister, Wilhelm; Blanchard, Laurence. "Letter to the Editor: Assignment of the 1H, 15N and 13C resonances of the
nucleocapsid-binding domain of the Sendai virus Phosphoprotein" J. Biomol. NMR 21, 75-76 (2001).
Assembly members:
X-protein of the sendai virus, polymer, 109 residues, 12719 Da.
Natural source: Common Name: Sendai virus (strain Harris) Taxonomy ID: 11196 Superkingdom: not available Kingdom: not available Genus/species: Paramyxovirus Sendai virus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
X-protein of the sendai virus: MRGSHHHHHHIEGRMKYKPD
LIREDEFRDEIRNPVYQERD
TEPRASNASRLLPSKEKPTM
HSLRLVIESSPLSRAEKAAY
VKSLSKCKTDQEVKAVMELV
EEDIESLTN
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 437 |
| 15N chemical shifts | 101 |
| 1H chemical shifts | 696 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | X-protein | 1 |
Entities:
Entity 1, X-protein 109 residues - 12719 Da.
| 1 | MET | ARG | GLY | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | ILE | GLU | GLY | ARG | MET | LYS | TYR | LYS | PRO | ASP | ||||
| 3 | LEU | ILE | ARG | GLU | ASP | GLU | PHE | ARG | ASP | GLU | ||||
| 4 | ILE | ARG | ASN | PRO | VAL | TYR | GLN | GLU | ARG | ASP | ||||
| 5 | THR | GLU | PRO | ARG | ALA | SER | ASN | ALA | SER | ARG | ||||
| 6 | LEU | LEU | PRO | SER | LYS | GLU | LYS | PRO | THR | MET | ||||
| 7 | HIS | SER | LEU | ARG | LEU | VAL | ILE | GLU | SER | SER | ||||
| 8 | PRO | LEU | SER | ARG | ALA | GLU | LYS | ALA | ALA | TYR | ||||
| 9 | VAL | LYS | SER | LEU | SER | LYS | CYS | LYS | THR | ASP | ||||
| 10 | GLN | GLU | VAL | LYS | ALA | VAL | MET | GLU | LEU | VAL | ||||
| 11 | GLU | GLU | ASP | ILE | GLU | SER | LEU | THR | ASN |
Samples:
sample_1: X-protein of the sendai virus, [U-15N; U-13C], 1.3 mM; potassium phosphate 50 mM; NaCl 0.5 M; DTT 10 mM
condition_1: ionic strength: 0.5 M; pH: 6.0; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | not available | not available | not available |
| MQ-HNCOCA | not available | not available | not available |
| MQ-COHNCA | not available | not available | not available |
| HNCA | not available | not available | not available |
| CBCANH | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| HNCO | not available | not available | not available |
| (HCA)CO(CA)NH | not available | not available | not available |
| H(CC)(CO)NH-TOCSY | not available | not available | not available |
| (H)CC(CO)NH-TOCSY | not available | not available | not available |
| HC(C)H-TOCSY | not available | not available | not available |
Software:
FELIX - data processing
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| DBJ | BAD74220 BAD74226 BAF73480 BAF73486 BAH59026 |
| EMBL | CAA24946 CAA34867 CAA34870 CAA34871 CAA34875 |
| GB | AAB06197 AAB06279 AAB06285 AAB06291 AAX07439 |
| SP | P04859 P04860 P14251 P14252 |
| AlphaFold | P14252 P04859 P04860 P14251 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks