BMRB Entry 4998

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4998

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Title:
1H, 13C and 15N Chemical Shift Assignments for the HPV-18 E2 DNA-binding Domain
Deposition date:
2001-04-25
Original release date:
2003-01-07
Authors:
Zhou, Casey; Mutchler, Veronica; McNab, Alistair; Garlick, Robert; Homa, Fred; Marshall, Vincent; Stockman, Brian
Citation:

Citation: Zhou, Casey; Mutchler, Veronica; McNab, Alistair; Garlick, Robert; Homa, Fred; Marshall, Vincent; Stockman, Brian. "1H, 13C and 15N Chemical Shift Assignments for the HPV-18 E2 DNA-binding Domain"  .

Assembly members:

Assembly members:
E2 DNA-binding domain, polymer, 89 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human papillomavirus Strain 18   Taxonomy ID: 10566   Superkingdom: not available   Kingdom: not available   Genus/species: Papillomavirus Human papillomavirus

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
E2 DNA-binding domain: MGNTTPIIHLKGDRNSLKCL RYRLRKHSDHYRDISSTWHW TGAGNEKTGILTVTYHSETQ RTKFLNTVAIPDSVQILVGY MTMHHHHHH

Data sets:
  • assigned_chemical_shifts
Data typeCount
1H chemical shifts441
13C chemical shifts291
15N chemical shifts76

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1E2-DBD monomer 11
2E2-DBD monomer 21

Entities:

Entity 1, E2-DBD monomer 1 89 residues - Formula weight is not available

1   METGLYASNTHRTHRPROILEILEHISLEU
2   LYSGLYASPARGASNSERLEULYSCYSLEU
3   ARGTYRARGLEUARGLYSHISSERASPHIS
4   TYRARGASPILESERSERTHRTRPHISTRP
5   THRGLYALAGLYASNGLULYSTHRGLYILE
6   LEUTHRVALTHRTYRHISSERGLUTHRGLN
7   ARGTHRLYSPHELEUASNTHRVALALAILE
8   PROASPSERVALGLNILELEUVALGLYTYR
9   METTHRMETHISHISHISHISHISHIS

Samples:

sample_1: E2 DNA-binding domain, [U-98% 13C; U-98% 15N], 0.8 mM

condition_1: pH: 6.5; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
HNCAsample_1not availablecondition_1
HN(CO)CAsample_1not availablecondition_1
CBCA(CO)NHsample_1not availablecondition_1
HBHA(CO)NHsample_1not availablecondition_1
HNCOsample_1not availablecondition_1
HCCH-TOCSYsample_1not availablecondition_1
H-N HSQCsample_1not availablecondition_1
H-N NOESY-HSQCsample_1not availablecondition_1

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
EMBL CAA28667
GB AAP20597 ABP99707 ABP99715 ABP99723 ABP99731
REF NP_040313
SP P06790
AlphaFold P06790

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks