BMRB Entry 4996

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4996
MolProbity Validation Chart

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Title:
NMR-Based Structure of the Conserved Protein MTH865 from the Archea Methanobacterium thermoautotrophicum
Deposition date:
2001-04-20
Original release date:
2001-10-08
Authors:
Lee, Gregory; Edwards, Aled; Arrowsmith, Cheryl; McIntosh, Lawrence
Citation:

Citation: Lee, Gregory; Edwards, Aled; Arrowsmith, Cheryl; McIntosh, Lawrence. "Letter to the Editor: NMR-Based Structure of the Conserved Protein MTH865 from the Archaeon Methanobacterium thermoautotrophicum"  J. Biomol. NMR 21, 63-66 (2001).

Assembly members:

Assembly members:
Methanobacterium thermoautotrophicum open reading frame 865, polymer, 84 residues, 8675 Da.

Natural source:

Natural source:   Common Name: Methanothermobacter thermautotrophicus   Taxonomy ID: 145262   Superkingdom: Archaea   Kingdom: Euryarchaeota   Genus/species: Methanothermobacter thermautotrophicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Methanobacterium thermoautotrophicum open reading frame 865: GSHMKMGVKEDIRGQIIGAL AGADFPINSPEELMAALPNG PDTTCKSGDVELKASDAGQV LTADDFPFKSAEEVADTIVN KAGL

Data sets:
  • assigned_chemical_shifts
Data typeCount
1H chemical shifts540
13C chemical shifts324
15N chemical shifts81

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1MTH8651

Entities:

Entity 1, MTH865 84 residues - 8675 Da.

1   GLYSERHISMETLYSMETGLYVALLYSGLU
2   ASPILEARGGLYGLNILEILEGLYALALEU
3   ALAGLYALAASPPHEPROILEASNSERPRO
4   GLUGLULEUMETALAALALEUPROASNGLY
5   PROASPTHRTHRCYSLYSSERGLYASPVAL
6   GLULEULYSALASERASPALAGLYGLNVAL
7   LEUTHRALAASPASPPHEPROPHELYSSER
8   ALAGLUGLUVALALAASPTHRILEVALASN
9   LYSALAGLYLEU

Samples:

15N-labeled_sample: Methanobacterium thermoautotrophicum open reading frame 865, [U-100% 15N], 0.5 mM

13C_15N-labeled_sample: Methanobacterium thermoautotrophicum open reading frame 865, [U-100% 13C; U-100% 15N], 0.5 mM

10%_13C-labeled_sample: Methanobacterium thermoautotrophicum open reading frame 865, [U-10% 13C; U-100% 15N], 0.5 mM

condition_1: pH: 6.51; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
500MHz Unity housed at Univ. of British Columbianot availablenot availablecondition_1
600MHz INOVA housed at Univ. of Torontonot availablenot availablecondition_1
800MHz INOVA housed at Univ. of Albertanot availablenot availablecondition_1

Software:

Felix v2000 - raw data processing, peak picking, peak assignments

NMR spectrometers:

  • Varian UNITY 500 MHz
  • Varian INOVA 600 MHz
  • Varian INOVA 800 MHz

Related Database Links:

PDB
DBJ BAM70032
EMBL CAA48863 CAA48865
GB AAB85363
REF NP_276002 WP_010876498

Download HSQC peak lists in one of the following formats:
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SPARKY: Backbone or all simulated peaks