BMRB Entry 4978
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4978
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Citation: Alexandrescu, Andrei; Maciejewski, Mark; Ruegg, Markus; Engel, Jurgen; Kammerer, Richard. "Letter to the Editor: 1H, 13C and 15N backbone assignments for the C-terminal
globular domain of agrin" J. Biomol. NMR 20, 295-296 (2001).
Assembly members:
agrin-G3, polymer, 195 residues, 21310 Da.
CA, non-polymer, 40.078 Da.
Natural source: Common Name: chicken Taxonomy ID: 9031 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Gallus gallus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGSTHis: a derivative of pGEX-1 (Amersham Pharmacia Biotech) that contains a 6xHis tag followed by a thrombin cleavage site between the gluthathione S-transferase (GST) carrier protein and the agrin-G3 domain.
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 532 |
| 13C chemical shifts | 548 |
| 15N chemical shifts | 193 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | agrin-G3 | 1 |
| 2 | Ca 2+ | 2 |
Entities:
Entity 1, agrin-G3 195 residues - 21310 Da.
| 1 | GLY | SER | GLU | LYS | VAL | ILE | ILE | GLU | LYS | ALA | ||||
| 2 | ALA | GLY | ASP | ALA | GLU | ALA | ILE | ALA | PHE | ASP | ||||
| 3 | GLY | ARG | THR | TYR | MET | GLU | TYR | HIS | ASN | ALA | ||||
| 4 | VAL | THR | LYS | SER | GLU | LYS | ALA | LEU | GLN | SER | ||||
| 5 | ASN | HIS | PHE | GLU | LEU | SER | ILE | LYS | THR | GLU | ||||
| 6 | ALA | THR | GLN | GLY | LEU | ILE | LEU | TRP | SER | GLY | ||||
| 7 | LYS | GLY | LEU | GLU | ARG | SER | ASP | TYR | ILE | ALA | ||||
| 8 | LEU | ALA | ILE | VAL | ASP | GLY | PHE | VAL | GLN | MET | ||||
| 9 | MET | TYR | ASP | LEU | GLY | SER | LYS | PRO | VAL | VAL | ||||
| 10 | LEU | ARG | SER | THR | VAL | PRO | ILE | ASN | THR | ASN | ||||
| 11 | HIS | TRP | THR | HIS | ILE | LYS | ALA | TYR | ARG | VAL | ||||
| 12 | GLN | ARG | GLU | GLY | SER | LEU | GLN | VAL | GLY | ASN | ||||
| 13 | GLU | ALA | PRO | ILE | THR | GLY | SER | SER | PRO | LEU | ||||
| 14 | GLY | ALA | THR | GLN | LEU | ASP | THR | ASP | GLY | ALA | ||||
| 15 | LEU | TRP | LEU | GLY | GLY | MET | GLU | ARG | LEU | SER | ||||
| 16 | VAL | ALA | HIS | LYS | LEU | PRO | LYS | ALA | TYR | SER | ||||
| 17 | THR | GLY | PHE | ILE | GLY | CYS | ILE | ARG | ASP | VAL | ||||
| 18 | ILE | VAL | ASP | ARG | GLN | GLU | LEU | HIS | LEU | VAL | ||||
| 19 | GLU | ASP | ALA | LEU | ASN | ASN | PRO | THR | ILE | LEU | ||||
| 20 | HIS | CYS | SER | ALA | LYS |
Entity 2, Ca 2+ - Ca - 40.078 Da.
| 1 | CA |
Samples:
sample_1: agrin-G3, [U-13C; U-15N; U-90% 2H], 1.0 mM; CaCl2 4 mM; immidazole 10 mM
sample_2: agrin-G3, [U-15N], 1.0 mM; CaCl2 4 mM; immidazole 10 mM
Ex-Cond_1: pH: 6.5; temperature: 298 K; pressure: 1 atm
Ex-Cond_2: pH: 7.1; temperature: 298 K; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HSQC-NOESY-HSQC (200 ms mix time) | not available | not available | not available |
| deuterium-decoupled TROSY versions of: | not available | not available | not available |
| 3D HNCACB | not available | not available | not available |
| HNCO | not available | not available | not available |
| HN(CA)CO | not available | not available | not available |
| 3D 15N TOCSY-HSQC (39 ms mix time) | not available | not available | not available |
| 3D 15N NOESY-HSQC (100 mix time) | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| PDB |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks