BMRB Entry 4966

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR4966
MolProbity Validation Chart

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Title:
1H and 13C Chemical Shift Assignments for Cardiotoxin A3 from Naja Atra at Neutral pH
Deposition date:
2001-03-06
Original release date:
2001-03-09
Authors:
Sue, Shih-Che; Jarrell, Harold; Brisson, Jean-Robert; Wu, Wen-guey
Citation:

Citation: Sue, Shih-Che; Jarrell, Harold; Brisson, Jean-Robert; Wu, Wen-guey. "Dynamic Characterization of the Water binding loop in the P-type Cardiotoxin: Implication for the role of the bound Water Molecule"  Biochemistry 40, 12782-12794 (2001).
PubMed: 11669614

Assembly members:

Assembly members:
Cardiotoxin A3, polymer, 60 residues, 6741 Da.

Natural source:

Natural source:   Common Name: Taiwan cobra   Taxonomy ID: 8656   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: cobra naja atra

Experimental source:

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Cardiotoxin A3: LKCNKLVPLFYKTCPAGKNL CYKMFMVATPKVPVKRGCID VCPKSSLLVKYVCCNTDRCN

Data sets:
Data typeCount
13C chemical shifts59
1H chemical shifts432

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1cardiotoxin A31

Entities:

Entity 1, cardiotoxin A3 60 residues - 6741 Da.

1   LEULYSCYSASNLYSLEUVALPROLEUPHE
2   TYRLYSTHRCYSPROALAGLYLYSASNLEU
3   CYSTYRLYSMETPHEMETVALALATHRPRO
4   LYSVALPROVALLYSARGGLYCYSILEASP
5   VALCYSPROLYSSERSERLEULEUVALLYS
6   TYRVALCYSCYSASNTHRASPARGCYSASN

Samples:

sample_1: Cardiotoxin A3 5 mM; phosphate buffer 10 mM

CTX_A3_condition: ionic strength: 0.03 M; pH: 5.96; pressure: 1.013 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
COSYnot availablenot availablenot available
NOESYnot availablenot availablenot available
TOCSYnot availablenot availablenot available
HSQCnot availablenot availablenot available

Software:

XWINNMR v2.01 -

NMR spectrometers:

  • BRUKER DRX 600 MHz
  • BRUKER DMX 500 MHz

Related Database Links:

BMRB 15305
PDB
EMBL CAA07686 CAA90963 CAB42053 CAB42055
GenBank AAA49386 AAB01541 AAB18382 AAB18383 AAB25733
PIR JK0222
PRF 0406231A 2207174B
SWISS-PROT O93471 O93473 P60301 P60302 P60303