BMRB Entry 4963

Title:
Chemical Shift Assignments and Coupling Constants for the rat Nedd4 WWIII domain - rat ENaC bP2 Peptide Complex
Deposition date:
2001-02-27
Original release date:
2001-04-30
Authors:
Kanelis, Voula; Rotin, Daniela; Forman-Kay, Julie
Citation:

Citation: Kanelis, Voula; Rotin, Daniela; Forman-Kay, Julie. "Chemical Shift Assignments and Coupling Constants for the rat Nedd4 WWIII domain - rat ENaC bP2 Peptide Complex"  Nat. Struct. Biol. 8, 407-412 (2001).

Assembly members:

Assembly members:
rat Nedd4 WWIII domain, polymer, 50 residues, 5659 Da.
rat epithelial Na+ Channel, bP2 region, polymer, 17 residues, 1726 Da.

Natural source:

Natural source:   Common Name: Norway rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX4T2

Experimental source:

Natural source:   Common Name: Norway rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX4T2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
rat Nedd4 WWIII domain: GSPVDSNDLGPLPPGWEERT HTDGRVFFINHNIKKTQWED PRMQNVAITG
rat epithelial Na+ Channel, bP2 region: GSTLPIPGTPPPNYDSL

Data typeCount
1H chemical shifts447
13C chemical shifts281
15N chemical shifts76
coupling constants89

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1rNedd4 WWIII domain1
2rENaC bP2 peptide2

Entities:

Entity 1, rNedd4 WWIII domain 50 residues - 5659 Da.

1   GLYSERPROVALASPSERASNASPLEUGLY
2   PROLEUPROPROGLYTRPGLUGLUARGTHR
3   HISTHRASPGLYARGVALPHEPHEILEASN
4   HISASNILELYSLYSTHRGLNTRPGLUASP
5   PROARGMETGLNASNVALALAILETHRGLY

Entity 2, rENaC bP2 peptide 17 residues - 1726 Da.

1   GLYSERTHRLEUPROILEPROGLYTHRPRO
2   PROPROASNTYRASPSERLEU

Samples:

Sample_1: rat Nedd4 WWIII domain, [U-13C; U-15N], 1.0 mM; rat epithelial Na+ Channel, bP2 region 3.0 mM; sodium phosphate 10 mM; EDTA 0.005 mM; aprotinin 0.01 mM; leupeptin 0.005 mM; AEBSF 0.005 mM; D2O 10%

Sample_2: rat Nedd4 WWIII domain, [U-13C; U-15N], 1.0 mM; rat epithelial Na+ Channel, bP2 region 3.0 mM; sodium phosphate 10 mM; EDTA 0.005 mM; aprotinin 0.01 mM; leupeptin 0.005 mM; AEBSF 0.005 mM; D2O 100%

Sample_3: rat epithelial Na+ Channel, bP2 region, [U-13C; U-15N], 1.0 mM; rat Nedd4 WWIII domain 3.0 mM; sodium phosphate 10 mM; EDTA 0.005 mM; aprotinin 0.01 mM; leupeptin 0.005 mM; AEBSF 0.005 mM; D2O 10%

Sample_4: rat epithelial Na+ Channel, bP2 region, [U-13C; U-15N], 1.0 mM; rat Nedd4 WWIII domain 3.0 mM; sodium phosphate 10 mM; EDTA 0.005 mM; aprotinin 0.01 mM; leupeptin 0.005 mM; AEBSF 0.005 mM; D2O 100%

Ex-cond_1: pH: 6.5; temperature: 303 K; ionic strength: 0.01 M; pressure: 1.01325 atm

Experiments:

NameSampleSample stateSample conditions
15N-hsqcnot availablenot availableEx-cond_1
13C-hsqc, aliphatic and aromatic regionsnot availablenot availableEx-cond_1
HNCOnot availablenot availableEx-cond_1
HNCaCbnot availablenot availableEx-cond_1
(Hb)CbCa(CO)NNHnot availablenot availableEx-cond_1
CCC-TOCSY-NNHnot availablenot availableEx-cond_1
HCC-TOCSY-NNHnot availablenot availableEx-cond_1
HCCH-TOCSY (sample 2 only)not availablenot availableEx-cond_1
HCCH-COSY (sample 1 only)not availablenot availableEx-cond_1
(Hb)CbCa(CO) N CaHa (sample 2 only)not availablenot availableEx-cond_1
HaCa N (sample 2 only)not availablenot availableEx-cond_1
15N/13C-edited NOESY-HSQC, 50 ms and 150 msnot availablenot availableEx-cond_1
HNHa-Jnot availablenot availableEx-cond_1
(Hb)Cb(CgCd)Hdnot availablenot availableEx-cond_1
(Hb)Cb(CgCd)Ce)Henot availablenot availableEx-cond_1
HMBC (sample 1 only)not availablenot availableEx-cond_1
HNCOCa COHaCa cross (sample 2 only)not availablenot availableEx-cond_1
HNCOCa HNHa cross (sample 2 only)not availablenot availableEx-cond_1
HaCaHb-J (sample 2 only)not availablenot availableEx-cond_1
HN(CO)Hb (sample 1 only)not availablenot availableEx-cond_1
HNHb (sample 1 only)not availablenot availableEx-cond_1
13C F1-filtered, F3-edited NOESY-HSQC, 250 msnot availablenot availableEx-cond_1
long range Jcc (sample 1 only)not availablenot availableEx-cond_1
HNCO-trosy (sample 1 only)not availablenot availableEx-cond_1

Software:

NMRPipe_NMRDraw v1.8 - NMR data processing

NMRView v4.1.2 - NMR data analysis, peak picking, semi-automated assignment

NMR spectrometers:

  • Varian Inova 500 MHz
  • Varian Inova 600 MHz
  • Varian Inova 800 MHz

Related Database Links:

PDB
EMBL CAA10572 CAA10572
GB EPQ17691
REF NP_001076244 XP_005882394 XP_006753700 XP_007986058 NP_001076244
SP O97742
SWISS-PROT O97742
AlphaFold O97742

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts