BMRB Entry 4963
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR4963
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Kanelis, Voula; Rotin, Daniela; Forman-Kay, Julie. "Chemical Shift Assignments and Coupling Constants for the
rat Nedd4 WWIII domain - rat ENaC bP2 Peptide Complex" Nat. Struct. Biol. 8, 407-412 (2001).
Assembly members:
rat Nedd4 WWIII domain, polymer, 50 residues, 5659 Da.
rat epithelial Na+ Channel, bP2 region, polymer, 17 residues, 1726 Da.
Natural source: Common Name: Norway rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX4T2
Entity Sequences (FASTA):
rat Nedd4 WWIII domain: GSPVDSNDLGPLPPGWEERT
HTDGRVFFINHNIKKTQWED
PRMQNVAITG
rat epithelial Na+ Channel, bP2 region: GSTLPIPGTPPPNYDSL
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 1H chemical shifts | 447 |
| 13C chemical shifts | 281 |
| 15N chemical shifts | 76 |
| coupling constants | 89 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | rNedd4 WWIII domain | 1 |
| 2 | rENaC bP2 peptide | 2 |
Entities:
Entity 1, rNedd4 WWIII domain 50 residues - 5659 Da.
| 1 | GLY | SER | PRO | VAL | ASP | SER | ASN | ASP | LEU | GLY | |
| 2 | PRO | LEU | PRO | PRO | GLY | TRP | GLU | GLU | ARG | THR | |
| 3 | HIS | THR | ASP | GLY | ARG | VAL | PHE | PHE | ILE | ASN | |
| 4 | HIS | ASN | ILE | LYS | LYS | THR | GLN | TRP | GLU | ASP | |
| 5 | PRO | ARG | MET | GLN | ASN | VAL | ALA | ILE | THR | GLY |
Entity 2, rENaC bP2 peptide 17 residues - 1726 Da.
| 1 | GLY | SER | THR | LEU | PRO | ILE | PRO | GLY | THR | PRO | ||||
| 2 | PRO | PRO | ASN | TYR | ASP | SER | LEU |
Samples:
Sample_1: rat Nedd4 WWIII domain, [U-13C; U-15N], 1.0 mM; rat epithelial Na+ Channel, bP2 region 3.0 mM; sodium phosphate 10 mM; EDTA 0.005 mM; aprotinin 0.01 mM; leupeptin 0.005 mM; AEBSF 0.005 mM; D2O 10%
Sample_2: rat Nedd4 WWIII domain, [U-13C; U-15N], 1.0 mM; rat epithelial Na+ Channel, bP2 region 3.0 mM; sodium phosphate 10 mM; EDTA 0.005 mM; aprotinin 0.01 mM; leupeptin 0.005 mM; AEBSF 0.005 mM; D2O 100%
Sample_3: rat epithelial Na+ Channel, bP2 region, [U-13C; U-15N], 1.0 mM; rat Nedd4 WWIII domain 3.0 mM; sodium phosphate 10 mM; EDTA 0.005 mM; aprotinin 0.01 mM; leupeptin 0.005 mM; AEBSF 0.005 mM; D2O 10%
Sample_4: rat epithelial Na+ Channel, bP2 region, [U-13C; U-15N], 1.0 mM; rat Nedd4 WWIII domain 3.0 mM; sodium phosphate 10 mM; EDTA 0.005 mM; aprotinin 0.01 mM; leupeptin 0.005 mM; AEBSF 0.005 mM; D2O 100%
Ex-cond_1: pH: 6.5; temperature: 303 K; ionic strength: 0.01 M; pressure: 1.01325 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N-hsqc | not available | not available | Ex-cond_1 |
| 13C-hsqc, aliphatic and aromatic regions | not available | not available | Ex-cond_1 |
| HNCO | not available | not available | Ex-cond_1 |
| HNCaCb | not available | not available | Ex-cond_1 |
| (Hb)CbCa(CO)NNH | not available | not available | Ex-cond_1 |
| CCC-TOCSY-NNH | not available | not available | Ex-cond_1 |
| HCC-TOCSY-NNH | not available | not available | Ex-cond_1 |
| HCCH-TOCSY (sample 2 only) | not available | not available | Ex-cond_1 |
| HCCH-COSY (sample 1 only) | not available | not available | Ex-cond_1 |
| (Hb)CbCa(CO) N CaHa (sample 2 only) | not available | not available | Ex-cond_1 |
| HaCa N (sample 2 only) | not available | not available | Ex-cond_1 |
| 15N/13C-edited NOESY-HSQC, 50 ms and 150 ms | not available | not available | Ex-cond_1 |
| HNHa-J | not available | not available | Ex-cond_1 |
| (Hb)Cb(CgCd)Hd | not available | not available | Ex-cond_1 |
| (Hb)Cb(CgCd)Ce)He | not available | not available | Ex-cond_1 |
| HMBC (sample 1 only) | not available | not available | Ex-cond_1 |
| HNCOCa COHaCa cross (sample 2 only) | not available | not available | Ex-cond_1 |
| HNCOCa HNHa cross (sample 2 only) | not available | not available | Ex-cond_1 |
| HaCaHb-J (sample 2 only) | not available | not available | Ex-cond_1 |
| HN(CO)Hb (sample 1 only) | not available | not available | Ex-cond_1 |
| HNHb (sample 1 only) | not available | not available | Ex-cond_1 |
| 13C F1-filtered, F3-edited NOESY-HSQC, 250 ms | not available | not available | Ex-cond_1 |
| long range Jcc (sample 1 only) | not available | not available | Ex-cond_1 |
| HNCO-trosy (sample 1 only) | not available | not available | Ex-cond_1 |
Software:
NMRPipe_NMRDraw v1.8 - NMR data processing
NMRView v4.1.2 - NMR data analysis, peak picking, semi-automated assignment
NMR spectrometers:
- Varian Inova 500 MHz
- Varian Inova 600 MHz
- Varian Inova 800 MHz
Related Database Links:
| PDB | |
| EMBL | CAA10572 CAA10572 |
| GB | EPQ17691 |
| REF | NP_001076244 XP_005882394 XP_006753700 XP_007986058 NP_001076244 |
| SP | O97742 |
| SWISS-PROT | O97742 |
| AlphaFold | O97742 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks