BMRB Entry 4960
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR4960
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
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Citation: Miller, Gregory; Hardman, John; Timkovich, Russell. "Solution Conformation of the Met 61 to His 61 Mutant of Pseudomonas stutzeri
ZoBell Foerrocytochrome c-551" Biophys. J. 80, .-. (2001).
Assembly members:
cytochrome c-551, polymer, 82 residues, 9500 Da.
HEC, non-polymer, 618.503 Da.
Natural source: Common Name: Pseudomonas stutzeri ZoBell Taxonomy ID: 96564 Superkingdom: Bacteria Kingdom: not available Genus/species: Pseudomonas stutzeri
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
cytochrome c-551: QDGEALFKSKPCAACHSVDT
KMVGPALKEVAAKNAGVEGA
ADTLALHIKNGSQGVWGPIP
HPPNPVTEEEAKILAEWVLS
LK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 509 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | M61H cyt c-551 | 1 |
| 2 | heme c | 2 |
Entities:
Entity 1, M61H cyt c-551 82 residues - 9500 Da.
| 1 | GLN | ASP | GLY | GLU | ALA | LEU | PHE | LYS | SER | LYS | ||||
| 2 | PRO | CYS | ALA | ALA | CYS | HIS | SER | VAL | ASP | THR | ||||
| 3 | LYS | MET | VAL | GLY | PRO | ALA | LEU | LYS | GLU | VAL | ||||
| 4 | ALA | ALA | LYS | ASN | ALA | GLY | VAL | GLU | GLY | ALA | ||||
| 5 | ALA | ASP | THR | LEU | ALA | LEU | HIS | ILE | LYS | ASN | ||||
| 6 | GLY | SER | GLN | GLY | VAL | TRP | GLY | PRO | ILE | PRO | ||||
| 7 | HIS | PRO | PRO | ASN | PRO | VAL | THR | GLU | GLU | GLU | ||||
| 8 | ALA | LYS | ILE | LEU | ALA | GLU | TRP | VAL | LEU | SER | ||||
| 9 | LEU | LYS |
Entity 2, heme c - C34 H34 Fe N4 O4 - 618.503 Da.
| 1 | HEC |
Samples:
literature_sample: cytochrome c-5514 – 5 mM; H2O 90%; D2O 10%; sodium borate buffer mM
literature_sample_conditions: pH: 9.2; temperature: 323 K; ionic strength: 0.15 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| NOESY | literature_sample | not available | literature_sample_conditions |
| DQF-COSY | literature_sample | not available | literature_sample_conditions |
| HOHAHA | literature_sample | not available | literature_sample_conditions |
Software:
FELIX v95 -
NMR spectrometers:
- Bruker AMX 500 MHz